Regional fractional ventilation mapping in spontaneously breathing mice using hyperpolarized 129 Xe MRI
The feasibility of ventilation imaging with hyperpolarized (HP) 129 Xe MRI has been investigated for quantitative and regional assessment of ventilation in spontaneously breathing mice. The multiple breath ventilation imaging technique was modified to the protocol of spontaneous inhalation of HP 129...
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Published in | NMR in biomedicine Vol. 28; no. 1; pp. 24 - 29 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2015
|
Online Access | Get full text |
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Summary: | The feasibility of ventilation imaging with hyperpolarized (HP)
129
Xe MRI has been investigated for quantitative and regional assessment of ventilation in spontaneously breathing mice. The multiple breath ventilation imaging technique was modified to the protocol of spontaneous inhalation of HP
129
Xe delivered continuously from a
129
Xe polarizer. A series of
129
Xe ventilation images was obtained by varying the number of breaths before the
129
Xe lung imaging. The fractional ventilation,
r
, was successfully evaluated for spontaneously breathing mice. An attempt was made to detect ventilation dysfunction in the emphysematous mouse lung induced by intratracheal administration of porcine pancreatic elastase (PPE). As a result, the distribution of fractional ventilation could be visualized by the
r
map. Significant dysfunction of ventilation was quantitatively identified in the PPE‐treated group. The whole‐lung
r
value of 0.34 ± 0.01 for control mice (
N
= 4) was significantly reduced, to 0.25 ± 0.07, in PPE‐treated mice (
N
= 4) (
p
= 0.038). This study is the first application of multiple breath ventilation imaging to spontaneously breathing mice, and shows that this methodology is sensitive to differences in the pulmonary ventilation. This methodology is expected to improve simplicity as well as noninvasiveness when assessing regional ventilation in small rodents. Copyright © 2014 John Wiley & Sons, Ltd. |
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ISSN: | 0952-3480 1099-1492 |
DOI: | 10.1002/nbm.3222 |