Comparison of Toxicity between Saxitoxin and Decarbamoyl Saxitoxin in the Mouse Bioassay for Paralytic Shellfish Poisoning Toxins

• Published in: Journal of Veterinary Medical Science Vol. 76; no. 11; pp. 1523 - 1525
• Main Authors: SUZUKI, Hodaka, MACHII, Kenji
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2014; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Biological Assay - methods; Biological Assay - standards; comparative toxicity; decarbamoyl saxitoxin (dcSTX); Japan; Laboratory Animal Science; Mice; mouse bioassay (MBA); Reference Standards; saxitoxin (STX); Saxitoxin - analogs & derivatives; Saxitoxin - toxicity; Shellfish - analysis; Shellfish Poisoning - diagnosis; Specific Pathogen-Free Organisms; Tissue Extracts - toxicity; Japan; INW, Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.14-0211

The mouse bioassay (MBA) for paralytic shellfish poisoning (PSP) toxins has been used in the AOAC Official Method and the official Japanese method. In the AOAC Official Method, the saxitoxin (STX) standard provided by the U.S. Food and Drug Administration (FDA) is used, but no standard is used in the official Japanese method. The objective of this study was to compare the toxicity of decarbamoyl STX (dcSTX), one of the derivatives of STX and a candidate standard for the MBA for PSP toxins in Japan, to that of FDA STX in the MBA platform. In this study, the toxicity of dcSTX was 918.0 ± 44.9 mouse units/µmol, and the relative toxicity ratio of dcSTX to FDA STX based on moles was 0.478.