Generation of canine dendritic cells from peripheral blood mononuclear cells

Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the pre...

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Published inJournal of Veterinary Medical Science Vol. 65; no. 6; pp. 663 - 669
Main Authors Yoshida, H. (Gifu Univ. (Japan). Faculty of Agriculture), Momoi, Y, Taga, N, Ide, K, Yamazoe, K, Iwasaki, T, Kudo, T
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.2003
Japan Science and Technology Agency
Subjects
Animals
Antigens, CD - analysis
BLOOD CELLS
canine
Cell Differentiation - drug effects
Cell Line
Cells, Cultured
Coculture Techniques
Cricetinae
Culture Media - chemistry
dendritic cell
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - immunology
Dendritic Cells - metabolism
DOGS
Esterases - metabolism
Flow Cytometry
Humans
IMMUNOTHERAPY
LECTINS
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - drug effects
PBMC
Phagocytosis
phytohemagglutinin
Phytohemagglutinins - pharmacology
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Abstract Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 × 106 of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.
AbstractList Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10 super(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.
Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.
Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 × 106 of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.
Author Kudo, T
Iwasaki, T
Taga, N
Momoi, Y
Ide, K
Yoshida, H. (Gifu Univ. (Japan). Faculty of Agriculture)
Yamazoe, K
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Snippet Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we...
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SubjectTerms Animals
Antigens, CD - analysis
BLOOD CELLS
canine
Cell Differentiation - drug effects
Cell Line
Cells, Cultured
Coculture Techniques
Cricetinae
Culture Media - chemistry
dendritic cell
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - immunology
Dendritic Cells - metabolism
DOGS
Esterases - metabolism
Flow Cytometry
Humans
IMMUNOTHERAPY
LECTINS
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - drug effects
PBMC
Phagocytosis
phytohemagglutinin
Phytohemagglutinins - pharmacology
Title Generation of canine dendritic cells from peripheral blood mononuclear cells
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https://www.ncbi.nlm.nih.gov/pubmed/12867724
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