Generation of canine dendritic cells from peripheral blood mononuclear cells

• Published in: Journal of Veterinary Medical Science Vol. 65; no. 6; pp. 663 - 669
• Main Authors: Yoshida, H. (Gifu Univ. (Japan). Faculty of Agriculture), Momoi, Y, Taga, N, Ide, K, Yamazoe, K, Iwasaki, T, Kudo, T
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.2003; Japan Science and Technology Agency
• Subjects: Animals; Antigens, CD - analysis; BLOOD CELLS; canine; Cell Differentiation - drug effects; Cell Line; Cells, Cultured; Coculture Techniques; Cricetinae; Culture Media - chemistry; dendritic cell; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - immunology; Dendritic Cells - metabolism; DOGS; Esterases - metabolism; Flow Cytometry; Humans; IMMUNOTHERAPY; LECTINS; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - drug effects; PBMC; Phagocytosis; phytohemagglutinin; Phytohemagglutinins - pharmacology
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.65.663

Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 × 106 of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.