Targeted isolation and cultivation of uncultivated bacteria by reverse genomics
Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical...
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Published in | Nature biotechnology Vol. 37; no. 11; pp. 1314 - 1321 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Nature Publishing Group
01.11.2019
Springer Nature |
Subjects | |
Online Access | Get full text |
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Summary: | Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidated. Here, we report an approach to capture specific microorganisms from complex communities into pure cultures using genome-informed antibody engineering. We apply our reverse genomics approach to isolate and sequence single cells and to cultivate three different species-level lineages of human oral Saccharibacteria (TM7). Using our pure cultures, we show that all three Saccharibacteria species are epibionts of diverse Actinobacteria. We also isolate and cultivate human oral SR1 bacteria, which are members of a lineage of previously uncultured bacteria. Reverse-genomics-enabled cultivation of microorganisms can be applied to any species from any environment and has the potential to unlock the isolation, cultivation and characterization of species from as-yet-uncultured branches of the microbial tree of life. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 USDOE National Science Foundation (NSF) AC05-00OR22725 National Institutes of Health (NIH) These authors contributed equally to this work present address: Department of Natural Sciences, Northwest Missouri State University, Maryville, MO, USA. Author Contributions M.P. conceived the study, designed and performed experiments, and analyzed data. K.L.C. and J.H.C. designed and performed experiments and analyzed data. M.B., A.G.C., S.J., D.K., Z.Y. designed and performed experiments. S.C. and J.P. performed protein sequence analyses and structure modelling. M.P., M.H., A.G. and E.L. recruited human subjects and collected oral samples. K.L.C. and M.P. wrote the manuscript. |
ISSN: | 1087-0156 1546-1696 |
DOI: | 10.1038/s41587-019-0260-6 |