The potential of organoids in toxicologic pathology: role of toxicologic pathologists in in vitro chemical hepatotoxicity assessment

The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human- and rat-derived hepatocytes; we focus on the liver organoid met...

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Published inJournal of Toxicologic Pathology Vol. 35; no. 3; pp. 225 - 235
Main Authors Yoshida, Toshinori, Kobayashi, Mio, Uomoto, Suzuka, Ohshima, Kanami, Hara, Emika, Katoh, Yoshitaka, Takahashi, Naofumi, Harada, Takanori, Usui, Tatsuya, Elbadawy, Mohamed, Shibutani, Makoto
Format Journal Article
LanguageEnglish
Published Tokyo JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY 01.01.2022
The Japanese Society of Toxicologic Pathology
Japan Science and Technology Agency
Japanese Society of Toxicologic Pathology
Subjects
Animal welfare
Cell differentiation
chemical hepatotoxicity
Drug development
Hepatocytes
Hepatotoxicity
in vitro
In vitro methods and tests
Liver
Organoids
Pathology
Pesticides
rat
Toxicity
toxicologic pathologist
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Summary:The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human- and rat-derived hepatocytes; we focus on the liver organoid method, which has been extensively studied in recent years, and discuss how toxicologic pathologists can use their knowledge and experience to contribute to the development of in vitro chemical hepatotoxicity assessment methods for drugs, pesticides, and chemicals. We also propose how toxicological pathologists should assess toxicity regarding the putative distribution of undifferentiated and differentiated cells in the organoid when liver organoids are observed in hematoxylin and eosin–stained specimens. This was done while considering the usefulness and limitations of in vitro studies for toxicologic pathology assessment.
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ISSN:0914-9198
1881-915X
1347-7404
DOI:10.1293/tox.2022-0017