Quantitative estimates of the cytoplasmic, PSD, and NMDAR-bound pools of CaMKII in dendritic spines

• Published in: Brain research Vol. 1419; pp. 46 - 52
• Main Authors: Feng, Bihua, Raghavachari, Sridhar, Lisman, John
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.10.2011; Elsevier
• Subjects: Actin; Animals; Biochemistry and metabolism; Biological and medical sciences; brain; Ca2+/calmodulin-dependent protein kinase II; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; Calmodulin; Central nervous system; cytoplasm; Cytoplasm - enzymology; Cytoplasm - ultrastructure; Dendritic Spines; Dendritic Spines - enzymology; Dendritic Spines - ultrastructure; enzymology; Fundamental and applied biological sciences. Psychology; Hippocampus; Hippocampus - enzymology; Hippocampus - ultrastructure; metabolism; Mice; mushrooms; Neurology; neurons; Post-Synaptic Density; Post-Synaptic Density - enzymology; Post-Synaptic Density - ultrastructure; Postsynaptic density; Primary Cell Culture; Rats; Receptors, N-Methyl-D-Aspartate; Receptors, N-Methyl-D-Aspartate - metabolism; Receptors, N-Methyl-D-Aspartate - ultrastructure; Spine; ultrastructure; Vertebrates: nervous system and sense organs; Postsynaptic density; Spine; Actin; Calmodulin; Ca2+/calmodulin-dependent protein kinase II; Ca 2+/calmodulin-dependent protein kinase II; calmodulin-dependent protein kinase; Calcium; Enzyme; Transferases; Protein; kinase II; Kinase; Dendritic spine; calmodulin-dependent protein; Ca; Cytoplasm

CaMKII plays a critical role in long-term potentiation (LTP). The kinase is a major component of the postsynaptic density (PSD); however, it is also contained in the spine cytoplasm. CaMKII can now be monitored optically in living neurons, and it is therefore important to understand the contribution...