Quantitative estimates of the cytoplasmic, PSD, and NMDAR-bound pools of CaMKII in dendritic spines

• Published in: Brain research Vol. 1419; pp. 46 - 52
• Main Authors: Feng, Bihua, Raghavachari, Sridhar, Lisman, John
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.10.2011; Elsevier
• Subjects: Actin; Animals; Biochemistry and metabolism; Biological and medical sciences; brain; Ca2+/calmodulin-dependent protein kinase II; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; Calmodulin; Central nervous system; cytoplasm; Cytoplasm - enzymology; Cytoplasm - ultrastructure; Dendritic Spines; Dendritic Spines - enzymology; Dendritic Spines - ultrastructure; enzymology; Fundamental and applied biological sciences. Psychology; Hippocampus; Hippocampus - enzymology; Hippocampus - ultrastructure; metabolism; Mice; mushrooms; Neurology; neurons; Post-Synaptic Density; Post-Synaptic Density - enzymology; Post-Synaptic Density - ultrastructure; Postsynaptic density; Primary Cell Culture; Rats; Receptors, N-Methyl-D-Aspartate; Receptors, N-Methyl-D-Aspartate - metabolism; Receptors, N-Methyl-D-Aspartate - ultrastructure; Spine; ultrastructure; Vertebrates: nervous system and sense organs; Postsynaptic density; Spine; Actin; Calmodulin; Ca2+/calmodulin-dependent protein kinase II; Ca 2+/calmodulin-dependent protein kinase II; calmodulin-dependent protein kinase; Calcium; Enzyme; Transferases; Protein; kinase II; Kinase; Dendritic spine; calmodulin-dependent protein; Ca; Cytoplasm
• ISSN: 0006-8993; 1872-6240; 1872-6240
• DOI: 10.1016/j.brainres.2011.08.051

CaMKII plays a critical role in long-term potentiation (LTP). The kinase is a major component of the postsynaptic density (PSD); however, it is also contained in the spine cytoplasm. CaMKII can now be monitored optically in living neurons, and it is therefore important to understand the contribution of the PSD and cytoplasmic pools to optical signals. Here, we estimate the size of these pools under basal conditions. From EM immunolabeling data, we calculate that the PSD/cytoplasmic ratio is ~5%. A second independent estimate is derived from measurements indicating that the average mushroom spine PSD contains 90 to 240 holoenzymes. A cytoplasmic concentration of 16μM (~2590 holoenzymes) in the spine can be estimated from the total measured CaMKII content of hippocampal tissue, the relative volume of different compartments, and the spine–dendrite ratio of CaMKII (2:1). These numbers yield a second estimate (6%) of the PSD/spine ratio in good agreement with the first. The CaMKII bound to the NMDAR is important because preventing the formation of this complex blocks LTP induction. We estimate that the percentage of spine CaMKII held active by binding to the NMDAR is ~0.2%. Implications of the high spine concentration of CaMKII (>100μM alpha subunits) and the small fraction within the PSD are discussed. Of particular note, the finding that the CaMKII signal in spines shows only transient activation (open state) after LTP induction is subject to the qualification that it does not reflect the small but important pool bound to the NMDAR. ► CaMKII is contained in both the spine cytoplasm and in the PSD. ► The fraction of CaMKII in the PSD under basal conditions is less than 10%. ► Formation of the complex of CaMKII with the NMDAR is necessary for LTP. ► The fraction of CaMKII activated by binding to the NMDAR is less than 1%. ► The small fraction of CaMKII activated by binding to the NMDAR is too small to detect by current methods.