Significant association of RNF213 p.R4810K, a moyamoya susceptibility variant, with coronary artery disease

• Published in: PloS one Vol. 12; no. 4; p. e0175649
• Main Authors: Morimoto, Takaaki, Mineharu, Yohei, Ono, Koh, Nakatochi, Masahiro, Ichihara, Sahoko, Kabata, Risako, Takagi, Yasushi, Cao, Yang, Zhao, Lanying, Kobayashi, Hatasu, Harada, Kouji H, Takenaka, Katsunobu, Funaki, Takeshi, Yokota, Mitsuhiro, Matsubara, Tatsuaki, Yamamoto, Ken, Izawa, Hideo, Kimura, Takeshi, Miyamoto, Susumu, Koizumi, Akio
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.04.2017; Public Library of Science (PLoS)
• Subjects: Adenosine Triphosphatases - genetics; Age; Aged; Aged, 80 and over; Alleles; Amino Acid Substitution; Angina; Angina pectoris; Angiogenesis; Angiography; Antibodies; Arteries; Asian Continental Ancestry Group - genetics; Aspartate aminotransferase; Biochemistry; Biology and Life Sciences; Brain; Cardiology; Cardiovascular disease; Cardiovascular diseases; Case-Control Studies; Cerebral blood flow; Cerebral infarction; Circulation; Control stability; Coronary artery disease; Coronary Artery Disease - genetics; Coronary heart disease; Coronary vessels; Creatine; Dehydrogenase; Dentistry; Diabetes; Diagnosis; Environmental factors; Environmental science; Enzymes; Family medical history; Female; Finger; Gene expression; Genetic aspects; Genetic Predisposition to Disease; Genomic instability; Health care; Health risks; Heart; Heart attacks; Heart diseases; Histopathology; Homozygosity; Hospitals; Humans; In vivo methods and tests; Incidence; Japan; Male; Medical imaging; Medicine; Medicine and Health Sciences; Metabolic disorders; Middle Aged; Minority & ethnic groups; Moyamoya disease; Moyamoya Disease - genetics; Mutation; Myocardial infarction; Neurosurgery; Obesity; Polymorphism, Single Nucleotide; Population; Population studies; Populations; Preventive medicine; Public health; Replication; Research and Analysis Methods; Risk Factors; Smoke; Statistical analysis; Stem cells; Stroke; Studies; Ubiquitin-Protein Ligases - genetics; University graduates; Vascular diseases; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0175649

The genetic architecture of coronary artery disease has not been fully elucidated, especially in Asian countries. Moyamoya disease is a progressive cerebrovascular disease that is reported to be complicated by coronary artery disease. Because most Japanese patients with moyamoya disease carry the p.R4810K variant of the ring finger 213 gene (RNF213), this may also be a risk factor for coronary artery disease; however, this possibility has never been tested. We genotyped the RNF213 p.R4810K variant in 956 coronary artery disease patients and 716 controls and tested the association between p.R4810K and coronary artery disease. We also validated the association in an independent population of 311 coronary artery disease patients and 494 controls. In the replication study, the p.R4810K genotypes were imputed from genome-wide genotyping data based on the 1000 Genomes Project. We used multivariate logistic regression analyses to adjust for well-known risk factors such as dyslipidemia and smoking habits. In the primary study population, the frequency of the minor variant allele was significantly higher in patients with coronary artery disease than in controls (2.04% vs. 0.98%), with an odds ratio of 2.11 (p = 0.017). Under a dominant model, after adjustment for risk factors, the association remained significant, with an odds ratio of 2.90 (95% confidence interval: 1.37-6.61; p = 0.005). In the replication study, the association was significant after adjustment for age and sex (odds ratio = 4.99; 95% confidence interval: 1.16-21.53; p = 0.031), although it did not reach statistical significance when further adjusted for risk factors (odds ratio = 3.82; 95% confidence interval: 0.87-16.77; p = 0.076). The RNF213 p.R4810K variant appears to be significantly associated with coronary artery disease in the Japanese population.