Label-Free and Continuous-Flow Ferrohydrodynamic Separation of HeLa Cells and Blood Cells in Biocompatible Ferrofluids

• Published in: Advanced functional materials Vol. 26; no. 22; pp. 3990 - 3998
• Main Authors: Zhao, Wujun, Zhu, Taotao, Cheng, Rui, Liu, Yufei, He, Jian, Qiu, Hong, Wang, Lianchun, Nagy, Tamas, Querec, Troy D., Unger, Elizabeth R., Mao, Leidong
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 14.06.2016
• Subjects: Biocompatibility; cell separation; cervical cancer; Channels; Devices; Enrichment; Ferrofluids; ferrohydrodynamic separation; Ferrohydrodynamics; microfluidics; Red blood cells; Separation
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.201503838

In this study, a label‐free, low‐cost, and fast ferrohydrodynamic cell separation scheme is demonstrated using HeLa cells (an epithelial cell line) and red blood cells. The separation is based on cell size difference, and conducted in a custom‐made biocompatible ferrofluid that retains the viability of cells during and after the assay for downstream analysis. The scheme offers moderate‐throughput (≈106 cells h−1 for a single channel device) and extremely high recovery rate (>99%) without the use of any label. It is envisioned that this separation scheme will have clinical applications in settings where rapid cell enrichment and removal of contaminating blood will improve efficiency of screening and diagnosis such as cervical cancer screening based on mixed populations in exfoliated samples. A label‐free, low‐cost, and fast method in biocompatible ferrofluids is developed to ferrohydrodynamically separate HeLa cells and red blood cells based on their size difference. It offers moderate‐throughput (≈106 cells h−1 for a single channel device) and extremely high recovery rate (>99 %) for the enrichment of HeLa cells from blood.