Distinctly different dynamics and kinetics of two steroid receptors at the same response elements in living cells

Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/...

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Bibliographic Details
Published inPloS one Vol. 9; no. 8; p. e105204
Main Authors Nenseth, Hatice Z, Dezitter, Xavier, Tesikova, Martina, Mueller, Florian, Klokk, Tove I, Hager, Gordon L, Saatcioglu, Fahri
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 18.08.2014
Public Library of Science (PLoS)
Subjects
Androgens
Binding sites
Biology and life sciences
Blotting, Western
Cancer
Cell Line
Chromatin Immunoprecipitation
Comparative analysis
Deoxyribonucleic acid
DNA
DNA methylation
Endocrinology
Fluorescence Recovery After Photobleaching
Gene expression
Genomes
Glucocorticoid receptors
Glucocorticoids
Humans
Kinetics
Medical research
Phosphorylation
Proteins
Receptors
Receptors, Androgen - genetics
Receptors, Androgen - metabolism
Receptors, Glucocorticoid - genetics
Receptors, Glucocorticoid - metabolism
Recovery (Medical)
Regulatory sequences
Response Elements - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA polymerase
Steroid hormone receptors
Transcription factors
France
Norway
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Summary:Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/2), binding site occupancy and the resulting temporal changes in transcription upon binding of two closely related steroid receptors, the androgen and glucocorticoid receptors (AR and GR), to their common hormone REs (HREs). We show that there are significant differences at all of these levels between AR and GR at the MMTV HRE when activated by their ligands. These data show that two TFs bound at the same RE can have significantly different modes of action that can affect their responses to environmental cues.
Bibliography:Competing Interests: The authors have declared that no competing interests exist.
Current address: Department of Biochemistry, Centre for Cancer Biomedicine, Institute for Cancer Research, the Norwegian Radium Hospital, Oslo University Hospital, Montebello, Oslo, Norway
Current address: University Lille Nord de France, Lille, France
Conceived and designed the experiments: HZN XD FS TIK. Performed the experiments: HZN XD MT. Analyzed the data: HZN FM FS. Contributed reagents/materials/analysis tools: FM GH FS. Wrote the paper: HZN MT FS.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0105204