Alteration of Stimulus-Specific Guard Cell Calcium Oscillations and Stomatal Closing in Arabidopsis det3 Mutant

• Published in: Science (American Association for the Advancement of Science) Vol. 289; no. 5488; pp. 2338 - 2342
• Main Authors: Allen, Gethyn J., Chu, Sarah P., Schumacher, Karin, Shimazaki, Chad T., Vafeados, Dionne, Kemper, Andrea, Hawke, Scott D., Tallman, Gary, Tsien, Roger Y., Harper, Jeffrey F., Chory, Joanne, Schroeder, Julian I.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 29.09.2000; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Abscisic Acid - pharmacology; Agronomy. Soil science and plant productions; Animal cells; Arabidopsis - cytology; Arabidopsis - genetics; Arabidopsis - physiology; Biological and medical sciences; Biology; Calcium; Calcium - metabolism; Calcium Signaling; Cell Membrane - metabolism; Cell membranes; Cells; Cellular biology; Cold Temperature; Cytology; Economic plant physiology; Endoplasmic Reticulum - metabolism; Evolution; Fundamental and applied biological sciences. Psychology; Genes, Plant; Genetic mutation; Guard cells; Hydrogen Peroxide - pharmacology; Membrane Potentials; Mutation; Oscillation; Oscillations; Oxidative Stress; Plant cells; Plant cells and tissues; Plant genetics; Plant Leaves - cytology; Plant Leaves - physiology; Plant physiology and development; Potassium - metabolism; Proton-Translocating ATPases - genetics; Proton-Translocating ATPases - metabolism; Reptiles & amphibians; Stomata; Vacuolar Proton-Translocating ATPases; Vacuoles - metabolism; Water and solutes. Absorption, translocation and permeability; Water relations, transpiration, stomata; Oxidative stress; Calcium; Enzyme; Adenosinetriphosphatase; Guard cell; Arabidopsis thaliana; Signal transduction; Cruciferae; Dicotyledones; Angiospermae; Stomatal movement; Oscillation; Hydrolases; Spermatophyta; Mutation; Experimental plant; Biological accumulation
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.289.5488.2338

Cytosolic calcium oscillations control signaling in animal cells, whereas in plants their importance remains largely unknown. In wild-type Arabidopsis guard cells abscisic acid, oxidative stress, cold, and external calcium elicited cytosolic calcium oscillations of differing amplitudes and frequencies and induced stomatal closure. In guard cells of the V-ATPase mutant det3, external calcium and oxidative stress elicited prolonged calcium increases, which did not oscillate, and stomatal closure was abolished. Conversely, cold and abscisic acid elicited calcium oscillations in det3, and stomatal closure occurred normally. Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure.