Phylogenetic analysis of the hemagglutinin (H) gene of canine distemper viruses isolated from wild masked palm civets (Paguma larvata)

• Published in: Journal of Veterinary Medical Science Vol. 66; no. 12; pp. 1575 - 1578
• Main Authors: Hirama, K. (Tokyo Univ. (Japan)), Goto, Y, Uema, M, Endo, Y, Miura, R, Kai, C
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.12.2004; Japan Science and Technology Agency
• Subjects: AGGLUTININS; Amino Acid Sequence; Animals; Base Sequence; canine distemper virus; DISTEMPER VIRUS; Distemper Virus, Canine - genetics; DNA Primers; hemagglutinin; Hemagglutinins - genetics; Japan; masked palm civets; MOLECULAR BIOLOGY; Molecular Sequence Data; PHYLOGENY; Sequence Alignment; Sequence Analysis, DNA; Species Specificity; VIVERRIDAE; Viverridae - virology; Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.66.1575

Hemagglutinin (H) gene of two CDV isolates, the Haku93 and Haku00 strains, from masked palm civets was molecularly analyzed. H genes of both two CDVs contained one open reading frame encoding 607 amino acids. Nucleotide and predicted amino acid sequences of H gene of the CDV Haku93 and Haku00 revealed high similarity to those of recent field isolates such as the Yanaka and Tanu96, while they showed limited identity to those of old vaccine strains. Potential N-linked glycosylation sites in both Haku93 and Haku00 were identical to other recent CDV isolates. Phylogenetic analysis revealed that the CDV strains derived from masked palm civets were classified into the group of recent Japanese CDV isolates.