Live Single-cell Metabolomics of Tryptophan and Histidine Metabolites in a Rat Basophil Leukemia Cell

• Published in: Analytical Sciences Vol. 24; no. 12; pp. 1525 - 1527
• Main Authors: MIZUNO, Hajime, TSUYAMA, Naohiro, DATE, Sachiko, HARADA, Takanori, MASUJIMA, Tsutomu
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2008; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Analytical Chemistry; Animals; Basophils - metabolism; Basophils - pathology; Cell Line, Tumor; Cell Survival; Chemistry; Histidine - metabolism; Leukemia - metabolism; Leukemia - pathology; Metabolomics; Nanotechnology; Rapid Communications; Rats; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Tryptophan - metabolism
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.24.1525

A direct and rapid metabolic analysis of a live single cell was performed by live single-cell video-mass spectrometry. The contents of the cytoplasm and a granule were sucked into a nano-electrospray ionization (nano-ESI) tip, and were directly introduced into a Q-TOF mass spectrometer by nano-spray after the addition of an ionization solvent. The metabolic pathways and the locations of tryptophan and histidine metabolites were traced by this method for a cultured rat basophil leukemia cell line (RBL-2H3). The t-values of detected peaks by a t-test between the different location, e.g. cytoplasm and a granule, revealed the molecular localization of each MS peak. A direct and quick metabolomic analysis of a living cell under simultaneous video-microscopic observations was innovated.