A Glimpse of Streptococcal Toxic Shock Syndrome from Comparative Genomics of S. suis 2 Chinese Isolates

• Published in: PloS one Vol. 2; no. 3; p. e315
• Main Authors: Chen, Chen, Tang, Jiaqi, Dong, Wei, Wang, Changjun, Feng, Youjun, Wang, Jing, Zheng, Feng, Pan, Xiuzhen, Liu, Di, Li, Ming, Song, Yajun, Zhu, Xinxing, Sun, Haibo, Feng, Tao, Guo, Zhaobiao, Ju, Aiping, Ge, Junchao, Dong, Yaqing, Sun, Wen, Jiang, Yongqiang, Wang, Jun, Yan, Jinghua, Yang, Huanming, Wang, Xiaoning, Gao, George F., Yang, Ruifu, Wang, Jian, Yu, Jun
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.03.2007; Public Library of Science (PLoS)
• Subjects: Analysis; Animal experimentation; Animals; Antiarthritic agents; Antigens; Arthritis; Chen Chen; China; Disease; Disease Models, Animal; Disease Outbreaks; DNA sequencing; DNA, Bacterial - genetics; Epidemiology; Etiology; Five year plans; Gene sequencing; Genetics and Genomics; Genome, Bacterial; Genomes; Genomics; Health aspects; Humans; Immunology; Infections; Infectious Diseases; Laboratories; Listeria; Medicine; Meningitis; Microbiology; Molecular modelling; Outbreaks; Oxidative stress; Pathogenesis; Pathogenicity; Pathogens; Polyamide-imides; Polymerase Chain Reaction; Proteins; Regulation; Science; Sensitivity and Specificity; Septic shock; Septicemia; Shock, Septic - epidemiology; Shock, Septic - microbiology; Streptococcal Infections - genetics; Streptococcal Infections - microbiology; Streptococcal toxic shock syndrome; Streptococcus; Streptococcus agalactiae; Streptococcus infections; Streptococcus pneumoniae; Streptococcus suis - genetics; Streptococcus suis - isolation & purification; Streptococcus suis - pathogenicity; Swine; Swine Diseases - microbiology; Toxic shock syndrome; Virulence; Xanthomonas campestris; Zoonoses; China; Beijing China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0000315

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different Chinese SS2 strains was undertaken. Comparative genomics accompanied by several lines of experiments, including experimental animal infection, PCR assay, and expression analysis, were utilized to further dissect a candidate pathogenicity island (PAI). Here we show, for the first time, a novel molecular insight into Chinese isolates of highly invasive SS2, which caused two large-scale human STSS outbreaks in China. A candidate PAI of approximately 89 kb in length, which is designated 89K and specific for Chinese SS2 virulent isolates, was investigated at the genomic level. It shares the universal properties of PAIs such as distinct GC content, consistent with its pivotal role in STSS and high virulence. To our knowledge, this is the first PAI candidate from S. suis worldwide. Our finding thus sheds light on STSS triggered by SS2 at the genomic level, facilitates further understanding of its pathogenesis and points to directions of development on some effective strategies to combat highly pathogenic SS2 infections.