Effects of Liver-Supplemented Food on the Development of Embryos in Mice

• Published in: Biological & Pharmaceutical Bulletin Vol. 26; no. 4; pp. 553 - 556
• Main Authors: Akase, Tomoko, Yamashina, Shohei, Akase, Tomohide, Onodera, Satoshi, Okuda, Hirokazu, Tashiro, Shin-ichi
• Format: Journal Article
• Language: English; Japanese
• Published: Tokyo The Pharmaceutical Society of Japan 2003; Pharmaceutical Society of Japan; Maruzen; Japan Science and Technology Agency
• Subjects: Animals; Biological and medical sciences; Cattle; Dietary Supplements; Embryonic and Fetal Development - drug effects; Embryonic and Fetal Development - physiology; Female; fetal abnormality; food supplement; Liver; Liver Extracts - pharmacology; Liver Extracts - toxicity; Male; Medical sciences; Mice; Mice, Inbred ICR; Pregnancy; retinol palmitate; vitamin A; Vitamin A - pharmacology; Vitamin A - toxicity; food supplement; vitamin A; retinol palmitate; liver; fetal abnormality
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.26.553

We examined whether dietary intake of cattle liver-supplemented food induces reproductive effects in dams and developmental effects in embryos in the mouse model. Seven groups of 19 to 35 female mice each were given either powdered food or the food supplemented with crude liver homogenate, its lipophilic component, the defatted liver homogenate or vitamin A (retinol palmitate) during a 25-d period spanning from a week prior to mating to gestation day 18 (GD18). Fetal mortality and incidence of external abnormalities of the fetuses whose dams were given the diet supplemented with the crude liver homogenate increased dose-dependently with an increase in the supplemented amount of the crude liver homogenate. On the other hand, the defatted liver homogenate did not induce any reproductive or teratological effect. The vitamin A (VA)-supplemented food (950 IU/5 g food as VA) induced approximately the same levels of the incidence of total external abnormalities appearing at the same affected regions or organs as the foods supplemented with the 700 mg crude liver homogenate (1029 IU/5 g food as VA) and its lipophilic component (950 IU/5 g food as VA). The content of VA (as 1029 IU/5 g food) in the crude liver homogenate was found to be approximately equal to that in the lipophilic component (950 IU/5 g food as VA). Therefore, it was concluded that VA plays an important role in induction of the lethal and teratogenic effects in the fetuses whose dams were given the powdered foods supplemented with the crude liver homogenate and its lipophilic component.