CD2 is a surface marker for mouse and rat spermatogonial stem cells

The spermatogonial stem cell (SSC) population in testis is small, and the lack of SSC markers has severely handicapped research on these cells. During our attempt to identify genes involved in SSC aging, we found that CD2 is expressed in cultured SSCs. Flow cytometric analysis and spermatogonial tra...

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Bibliographic Details
Published inJournal of Reproduction and Development Vol. 66; no. 4; pp. 341 - 349
Main Authors KANATSU-SHINOHARA, Mito, CHEN, Guiying, MORIMOTO, Hiroko, SHINOHARA, Takashi
Format Journal Article
LanguageEnglish
Published Japan The Society for Reproduction and Development 2020
Japan Science and Technology Agency
Subjects
Aging
Apoptosis
CD2
Flow cytometry
Original
Spermatogonial stem cells
Stem cell transplantation
Stem cells
Surface markers
Testes
Transplantation
CD2
Spermatogonial stem cells
Transplantation
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Summary:The spermatogonial stem cell (SSC) population in testis is small, and the lack of SSC markers has severely handicapped research on these cells. During our attempt to identify genes involved in SSC aging, we found that CD2 is expressed in cultured SSCs. Flow cytometric analysis and spermatogonial transplantation experiments showed that CD2 is expressed in SSCs from mature adult mouse testes. Cultured SSCs transfected with short hairpin RNAs (shRNAs) against CD2 proliferated poorly and showed an increased frequency of apoptosis. Moreover, functional analysis of transfected cells revealed impairment of SSC activity. Fluorescence activated cell sorting and spermatogonial transplantation experiments showed that CD2 is expressed not only in mouse but also in rat SSCs. The results indicate that CD2 is a novel SSC surface marker conserved between mouse and rat SSCs.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.2020-019