Optimization of hydrogen peroxide detection for a methyl mercaptan biosensor

• Published in: Sensors (Basel, Switzerland) Vol. 13; no. 4; pp. 5028 - 5039
• Main Authors: Li, Zhan-Hong, Guedri, Houssemeddine, Viguier, Bruno, Sun, Shi-Gang, Marty, Jean-Louis
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.04.2013; Molecular Diversity Preservation International (MDPI)
• Subjects: amperometric sensor; Biosensing Techniques - instrumentation; Biosensors; Carbon; Catalysis; Chemistry; Cobalt; Electrochemical Techniques; Electrodes; Electrons; Ferrocyanides - chemistry; Horseradish Peroxidase - metabolism; Hydrogen peroxide; Hydrogen Peroxide - analysis; Indoles - chemistry; methyl mercaptan; Nanotubes, Carbon - chemistry; Organometallic Compounds - chemistry; Osmium - chemistry; Oxidation-Reduction; Potassium; screen printed electrode; Sensors; Sulfhydryl Compounds - analysis; Sulfur; United Kingdom > UK; France
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s130405028

Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H₂O₂) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H₂O₂, applying a potential of +0.6 V, +0.6 V, +0.4 V, -0.2 V and -0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H₂O₂ were 3.1 μM, 1.3 μM, 71 nM, 1.3 μM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H₂O₂ at a working potential as low as -0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 μM.