Gene Expression Profile of Aging and Its Retardation by Caloric Restriction

• Published in: Science (American Association for the Advancement of Science) Vol. 285; no. 5432; pp. 1390 - 1393
• Main Authors: Lee, Cheol-Koo, Klopp, Roger G., Weindruch, Richard, Prolla, Tomas A.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 27.08.1999; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Adults; Aging; Aging (Biology); Aging - genetics; Analysis; Animals; Biological and medical sciences; Biosynthesis; Caloric restriction; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Diet; DNA damage; DNA Damage - genetics; DNA repair; DNA Repair - genetics; Energy Intake; Energy metabolism; Energy Metabolism - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Genes; Genetic research; Laboratory animals; Logical Thinking; Male; Metabolism; Mice; Mice, Inbred C57BL; Mitochondria, Muscle - metabolism; Molecular and cellular biology; Muscle, Skeletal - innervation; Muscle, Skeletal - metabolism; Nutrition; Oligonucleotide Array Sequence Analysis; Oxidative Stress - genetics; Protein metabolism; Proteins - metabolism; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Scientific Concepts; Skeletal muscle; United States; Vertebrata; Senescence; Mammalia; Gene; Mouse; Differential gene expression; Ageing; Rodentia; Gene expression; Striated muscle; Restricted diet
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.285.5432.1390

The gene expression profile of the aging process was analyzed in skeletal muscle of mice. Use of high-density oligonucleotide arrays representing 6347 genes revealed that aging resulted in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes. Most alterations were either completely or partially prevented by caloric restriction, the only intervention known to retard aging in mammals. Transcriptional patterns of calorie-restricted animals suggest that caloric restriction retards the aging process by causing a metabolic shift toward increased protein turnover and decreased macromolecular damage.