Serologic and molecular evidence for circulation of Crimean-Congo hemorrhagic fever virus in ticks and cattle in Zambia

• Published in: PLoS neglected tropical diseases Vol. 15; no. 6; p. e0009452
• Main Authors: Kajihara, Masahiro, Simuunza, Martin, Saasa, Ngonda, Dautu, George, Mori-Kajihara, Akina, Qiu, Yongjin, Nakao, Ryo, Eto, Yoshiki, Furumoto, Hayato, Hang’ombe, Bernard M., Orba, Yasuko, Sawa, Hirofumi, Simulundu, Edgar, Fukushi, Shuetsu, Morikawa, Shigeru, Saijo, Masayuki, Arikawa, Jiro, Kabilika, Swithine, Monze, Mwaka, Mukonka, Victor, Mweene, Aaron, Takada, Ayato, Yoshimatsu, Kumiko
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 01.06.2021; Public Library of Science (PLoS)
• Subjects: Animals; Annealing; Arachnids; Biology and Life Sciences; Cattle; Computer and Information Sciences; Crimean hemorrhagic fever; Disease transmission; Distribution; Epidemiology; Fever; Genetic aspects; Genetic diversity; Genetic variation; Genomes; Genotype; Haemorrhage; Health aspects; Hemorrhage; Identification and classification; Immunofluorescence; Immunoglobulin G; Infections; Infectious diseases; Laboratories; Medicine and health sciences; Methods; Microbiological strains; People and Places; Phylogeny; Risk assessment; RNA polymerase; Sentinel health events; Serum; Strains; Ticks; Transmission; Tropical diseases; Vertebrates; Veterinary medicine; Virus-vector relationships; Viruses; Zoonoses; Zambia
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0009452

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne zoonosis with a high case fatality rate in humans. Although the disease is widely found in Africa, Europe, and Asia, the distribution and genetic diversity of CCHF virus (CCHFV) are poorly understood in African countries. To assess the risks of CCHF in Zambia, where CCHF has never been reported, epidemiologic studies in cattle and ticks were conducted. Through an indirect immunofluorescence assay, CCHFV nucleoprotein-specific serum IgG was detected in 8.4% (88/1,047) of cattle. Among 290 Hyalomma ticks, the principal vector of CCHFV, the viral genome was detected in 11 ticks. Phylogenetic analyses of the CCHFV S and M genome segments revealed that one of the detected viruses was a genetic reassortant between African and Asian strains. This study provides compelling evidence for the presence of CCHFV in Zambia and its transmission to vertebrate hosts.