Bacteriophage lambda display systems: developments and applications

• Published in: Applied microbiology and biotechnology Vol. 98; no. 7; pp. 2853 - 2866
• Main Authors: Nicastro, Jessica, Sheldon, Katlyn, Slavcev, Roderick A
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2014; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Bacteria; Bacteriophage lambda - genetics; Bacteriophages; Biomedical and Life Sciences; Biotechnology; Cell Surface Display Techniques - methods; coat proteins; drug delivery systems; gene transfer; Genetic aspects; Genetic engineering; Genetic recombination; Genetic research; Genetics; Genomes; Hepatitis C; Life Sciences; Membrane Proteins - biosynthesis; Membrane Proteins - genetics; Microbial Genetics and Genomics; Microbiology; Mini-Review; Molecular genetics; peptide libraries; Phage l; Proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Technology application; Vaccines; Viral proteins; Viral Structural Proteins - biosynthesis; Viral Structural Proteins - genetics; United Kingdom; Gene delivery; Phage vaccine; gpD translational fusion; Bacteriophage λ; Lytic phage display
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-014-5521-1

Bacteriophage (phage) Lambda (λ) has played a key historic role in driving our understanding of molecular genetics. The lytic nature of λ and the conformation of its major capsid protein gpD in capsid assembly offer several advantages as a phage display candidate. The unique formation of the λ capsid and the potential to exploit gpD in the design of controlled phage decoration will benefit future applications of λ display where steric hindrance and avidity are of great concern. Here, we review the recent developments in phage display technologies with phage λ and explore some key applications of this technology including vaccine delivery, gene transfer, bio-detection, and bio-control.