Hyperion Image Analysis Depicts a Preliminary Landscape of Tumor Immune Microenvironment in OSCC with Lymph Node Metastasis

• Published in: Journal of Immunology Research Vol. 2021; pp. 9975423 - 7
• Main Authors: Xie, Shang, Zhang, Xin-Yuan, Shan, Xiao-Feng, Yau, Vicky, Zhang, Jian-Yun, Wang, Wei, Yan, Yong-Pan, Cai, Zhi-Gang
• Format: Journal Article
• Language: English
• Published: Egypt Hindawi 2021; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Adult; Antibodies; B cells; Cancer therapies; CD8 antigen; Chemotherapy; Collagen; Dissection; Extracellular matrix; Female; Formaldehyde; Humans; Image processing; Image Processing, Computer-Assisted; Immunology; Labeling; Laser Scanning Cytometry - instrumentation; Lymph nodes; Lymphatic Metastasis - diagnostic imaging; Lymphatic Metastasis - immunology; Lymphatic Metastasis - pathology; Lymphatic system; Lymphocytes T; Male; Metastases; Metastasis; Microenvironments; Mouth Mucosa - diagnostic imaging; Mouth Mucosa - immunology; Mouth Mucosa - pathology; Mouth Neoplasms - diagnosis; Mouth Neoplasms - immunology; Mouth Neoplasms - pathology; Mouth Neoplasms - surgery; Neoplasia; Oral cancer; Oral squamous cell carcinoma; Paraffin; Patients; Prognosis; Radiation therapy; Squamous cell carcinoma; Squamous Cell Carcinoma of Head and Neck - diagnosis; Squamous Cell Carcinoma of Head and Neck - immunology; Squamous Cell Carcinoma of Head and Neck - pathology; Squamous Cell Carcinoma of Head and Neck - surgery; T cells; Tongue; Tumor Microenvironment - immunology; Tumors; Viral antibodies
• ISSN: 2314-8861; 2314-7156
• DOI: 10.1155/2021/9975423

Background. Oral squamous cell carcinoma (OSCC) constitutes the most common types of oral cancer. Because its prognosis varies significantly, identification of a tumor immune microenvironment could be a critical tool for treatment planning and predicting a more accurate prognosis. This study is aimed at utilizing the Hyperion imaging system to depict a preliminary landscape of the tumor immune microenvironment in OSCC with lymph node metastasis. Methods. We collected neoplasm samples from OSCC patients. Their formalin-fixed, paraffin-embedded (FFPE) tissue sections were obtained and stained utilizing a panel of 26 clinically relevant metal-conjugated antibodies. Detection and analysis were performed for these stained cells with the Hyperion imaging system. Results. Four patients met our inclusion criteria. We depicted a preliminary landscape of their tumor immune microenvironment and identified 25 distinct immune cell subsets from these OSCC patients based on phenotypic similarity. All these patients had decreased expression of CD8+ T cells in tumor specimens. Variety in cell subsets was seen, and more immune activated cells were found in patient A and patient B than those in patient C and patient D. Such differences in tumor immune microenvironments can contribute to forecasting of individual prognoses. Conclusion. The Hyperion imaging system helped to delineate a preliminary and multidimensional landscape of the tumor immune microenvironment in OSCC with lymph node metastasis and provided insights into the influence of the immune microenvironment in determination of prognoses. These results reveal possible contributory factors behind different prognoses of OSCC patients with lymph node metastasis and provide reference for individual treatment planning.