Identification of a Cullin Homology Region in a Subunit of the Anaphase- Promoting Complex

• Published in: Science (American Association for the Advancement of Science) Vol. 279; no. 5354; pp. 1219 - 1222
• Main Authors: Yu, Hongtao, Peters, Jan-Michael, King, Randall W., Page, Andrew M., Hieter, Philip, Kirschner, Marc W.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 20.02.1998; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Alleles; Amino Acid Sequence; Anaphase; Anaphase-Promoting Complex-Cyclosome; Animals; Antibodies; Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome; Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome; Apc4 Subunit, Anaphase-Promoting Complex-Cyclosome; Apc5 Subunit, Anaphase-Promoting Complex-Cyclosome; Apc7 Subunit, Anaphase-Promoting Complex-Cyclosome; Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome; Biological and medical sciences; Cell cycle; Cell Cycle - physiology; Cell Cycle Proteins - chemistry; Cell cycle, cell proliferation; Cell division; Cell physiology; Cellular biology; Climate; Cloning, Molecular; Coding; Cullin Proteins; Cyclins; Fundamental and applied biological sciences. Psychology; Genes; Helminth Proteins - chemistry; Humans; Ligases - chemistry; Ligases - genetics; Ligases - metabolism; Molecular and cellular biology; Molecular Sequence Data; Mutation; Open Reading Frames; Phylogeny; Physiological aspects; Plasmids; Polymerase chain reaction; Proteins - chemistry; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Saccharomycetales; Sequence Alignment; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins; Yeast; Yeasts; Yeast; Nucleotide sequence; Mitosis; Homology; Subunit; Fungi; Regulation(control); Gene; Cell cycle; Ascomycetes; Molecular complex; Mutation; Anaphase; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.279.5354.1219

The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratrico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G$_1$ phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.