Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2

• Published in: Emerging infectious diseases Vol. 27; no. 7; pp. 1811 - 1820
• Main Authors: Gultom, Mitra, Licheri, Matthias, Laloli, Laura, Wider, Manon, Strassle, Marina, Vkovski, Philip, Steiner, Silvio, Kratzel, Annika, Thao, Tran Thi Nhu, Probst, Lukas, Stalder, Hanspeter, Portmann, Jasmine, Holwerda, Melle, Ebert, Nadine, Stokar-Regenscheit, Nadine, Gurtner, Corinne, Zanolari, Patrik, Posthaus, Horst, Schuller, Simone, Vicente-Santos, Amanda, Moreira-Soto, Andres, Corrales-Aguilar, Eugenia, Ruggli, Nicolas, Tekes, Gergely, von Messling, Veronika, Sawatsky, Bevan, Thiel, Volker, Dijkman, Ronald
• Format: Journal Article
• Language: English
• Published: Atlanta U.S. National Center for Infectious Diseases 01.07.2021; Centers for Disease Control and Prevention
• Subjects: Animal experimentation; animal experimentation ethics; Animal species; Animals; Cattle; Cell culture; coronavirus disease; Coronaviruses; COVID-19; disease reservoirs; Domestic animals; epidemics; Epithelial cells; Genomes; Global health; Health aspects; Infections; Influenza; Medical ethics; Methods; Original Research; outbreaks; Pandemics; Polyclonal antibodies; Proteins; Respiratory diseases; RNA polymerase; Severe acute respiratory syndrome coronavirus 2; Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2; Viruses; Wildlife; Zoonoses; Switzerland; United States > US; Oklahoma
• ISSN: 1080-6040; 1080-6059
• DOI: 10.3201/eid2707.204660

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally, and the number of worldwide cases continues to rise. The zoonotic origins of SARS-CoV-2 and its intermediate and potential spillback host reservoirs, besides humans, remain largely unknown. Because of ethical and experimental constraints and more important, to reduce and refine animal experimentation, we used our repository of well-differentiated airway epithelial cell (AEC) cultures from various domesticated and wildlife animal species to assess their susceptibility to SARS-CoV-2. We observed that SARS-CoV-2 replicated efficiently only in monkey and cat AEC culture models. Whole-genome sequencing of progeny viruses revealed no obvious signs of nucleotide transitions required for SARS-CoV-2 to productively infect monkey and cat AEC cultures. Our findings, together with previous reports of human-to-animal spillover events, warrant close surveillance to determine the potential role of cats, monkeys, and closely related species as spillback reservoirs for SARS-CoV-2.