Isolation of Inhibitors of Adenylate Cyclase from Dan-shen, the Root of Salvia miltiorrhiza

• Published in: Chemical & pharmaceutical bulletin Vol. 37; no. 5; pp. 1287 - 1290
• Main Authors: YAMASAKI, Kazuo, TAKEDA, Osamu, YAMASHITA, Atsushi, KOHDA, Hiroshi, TANAKA, Seiji, KUROKAWA, Tomonori, ISHIBASHI, Sadahiko
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1989; 公益社団法人日本薬学会; Maruzen; Japan Science and Technology Agency
• Subjects: adenylate cyclase; Adenylyl Cyclase Inhibitors; Adenylyl Cyclases - blood; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; dan-shen; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - pharmacology; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; lithospermic acid; Lyases; Male; Rats; Rats, Inbred Strains; roots; rosemarinic acid; Salvia miltiorrhiza; Brain; Root; Rat; Enzyme; Rodentia; HPLC chromatography; Red blood cell; Enzyme inhibitor; NMR spectrometry; Biological activity; Dose activity relation; Adenylate cyclase; Vertebrata; Mammalia; Labiatae; Dicotyledones; Angiospermae; Isolation; Spermatophyta; Salvia
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.37.1287

The effect of dan-shen extract, the root of Salvia miltiorrhiza, on adenylate cyclase was investigated in both rat brain and rat erythrocytes. The EtOAc fraction of the MeOH extract was proved to have significant inhibitory activity. Potent inhibitory principles in the EtOAc fraction were isolated and identified as 4 polyphenolic acids, rosemarinic acid, lithospermic acid, and their methyl ester derivatives.