Initial cytotoxicity assays of media for sulfate-reducing bacteria: An endodontic biopharmaceutical product under development

• Published in: Dental Materials Journal Vol. 35; no. 5; pp. 762 - 768
• Main Authors: HEGGENDORN, Fabiano Luiz, Gabriela Cristina de Carvalho SILVA, CARDOSO, Elisama Azevedo, CASTRO, Helena Carla, GONÇALVES, Lúcio Souza, DIAS, Eliane Pedra, Viviane de Oliveira Freitas LIONE, LUTTERBACH, Márcia Teresa Soares
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japanese Society for Dental Materials and Devices 2016; Japanese Society for Dental Materials and Devices
• Subjects: Bacteria; Biocompatibility; Biotechnology; Cell Line; Cell Survival; Culture; Culture Media; Cytotoxicity; Dentistry; Endodontic; Endodontics; Exposure; Media; Medical device; Sulfates; Sulfur-Reducing Bacteria; Toxicity; Vehicles
• ISSN: 0287-4547; 1881-1361
• DOI: 10.4012/dmj.2015-360

This study assessed the cell viability of the inoculation vehicle of BACCOR (a combination of sulfate-reducing bacteria plus a culture media for bacteria), a biopharmaceutical product under development for dental use as aid in fractured endodontic file removal from the root canal. Different culture media for bacteria were evaluated: modified Postgate E (MCP-E mod), Modified Postgate E without Agar-agar (MCP-E w/Ag), Postgate C with Agar-agar (MCP-C Ag) and Postgate C without Agar-agar (MCP-C w/Ag). Cytotoxicity was quantified by the MTT test, exposing L929 and Vero cell lines to the vehicles over 24 h. The exposure of L929 cell line to MCP-E w/Ag resulted in biocompatibility (52% cell viability), while the exposure of the Vero kidney line revealed only MCP-E mod as cytotoxic. When diluted, all the vehicles showed biocompatibility with both cell lines. MCP-E w/Ag was the vehicle chosen for BACCOR, because of its biocompatibility with the cells used.