Activation of plant immunity by exposure to dinitrogen pentoxide gas generated from air using plasma technology

• Published in: PloS one Vol. 17; no. 6; p. e0269863
• Main Authors: Tsukidate, Daiki, Takashima, Keisuke, Sasaki, Shota, Miyashita, Shuhei, Kaneko, Toshiro, Takahashi, Hideki, Ando, Sugihiro
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 24.06.2022; Public Library of Science (PLoS)
• Subjects: Acids; Agriculture; Analysis; Antiinfectives and antibacterials; Antimicrobial peptides; Apoptosis; Arabidopsis thaliana; Atmospheric pressure; Biology and Life Sciences; Disease control; Disease resistance; Exposure; Gene expression; Genes; Health aspects; Humidity; Immunity; Jasmonic acid; Metabolism; Nitrogen; Nitrogen dioxide; Peptides; Pesticides; Physical Sciences; Physiology; Plant diseases; Plant immunity; Plant virus diseases; Plasma; Propagation; Reactive nitrogen species; Research and Analysis Methods; RNA sequencing; Signaling; Technology; Tomatoes; Tryptophan; Viruses; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0269863

Reactive nitrogen species (RNS) play an important role in plant immunity as signaling factors. We previously developed a plasma technology to partially convert air molecules into dinitrogen pentoxide (N.sub.2 O.sub.5 ), an RNS whose physiological action is poorly understood. To reveal the function of N.sub.2 O.sub.5 gas in plant immunity, Arabidopsis thaliana was exposed to plasma-generated N.sub.2 O.sub.5 gas once (20 s) per day for 3 days, and inoculated with Botrytis cinerea, Pseudomonas syringae pv. tomato DC3000 (Pst), or cucumber mosaic virus strain yellow (CMV(Y)) at 24 h after the final N.sub.2 O.sub.5 gas exposure. Lesion size with B. cinerea infection was significantly (P < 0.05) reduced by exposure to N.sub.2 O.sub.5 gas. Propagation of CMV(Y) was suppressed in plants exposed to N.sub.2 O.sub.5 gas compared with plants exposed to the air control. However, proliferation of Pst in the N.sub.2 O.sub.5 -gas-exposed plants was almost the same as in the air control plants. These results suggested that N.sub.2 O.sub.5 gas exposure could control plant disease depending on the type of pathogen. Furthermore, changes in gene expression at 24 h after the final N.sub.2 O.sub.5 gas exposure were analyzed by RNA-Seq. Based on the gene ontology analysis, jasmonic acid and ethylene signaling pathways were activated by exposure of Arabidopsis plants to N.sub.2 O.sub.5 gas. A time course experiment with qRT-PCR revealed that the mRNA expression of the transcription factor genes, WRKY25, WRKY26, WRKY33, and genes for tryptophan metabolic enzymes, CYP71A12, CYP71A13, PEN2, and PAD3, was transiently induced by exposure to N.sub.2 O.sub.5 gas once for 20 s peaking at 1-3 h post-exposure. However, the expression of PDF1.2 was enhanced beginning from 6 h after exposure and its high expression was maintained until 24-48 h later. Thus, enhanced tryptophan metabolism leading to the synthesis of antimicrobial substances such as camalexin and antimicrobial peptides might have contributed to the N.sub.2 O.sub.5 -gas-induced disease resistance.