Ploidy Regulation of Gene Expression

• Published in: Science (American Association for the Advancement of Science) Vol. 285; no. 5425; pp. 251 - 254
• Main Authors: Galitski, Timothy, Saldanha, Alok J., Styles, Cora A., Lander, Eric S., Fink, Gerald R.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 09.07.1999; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Biological and medical sciences; Cell cycle; Cells; Chitinases - genetics; Classical genetics, quantitative genetics, hybrids; Cyclins - genetics; Diploidy; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; G1 Phase; Gene expression; Gene expression regulation; Gene Expression Regulation, Fungal; Genes; Genetics of eukaryotes. Biological and molecular evolution; Haploidy; Lipoproteins - genetics; Lipoproteins - physiology; Mating Factor; Membrane Glycoproteins; Membrane Proteins - genetics; Mother cells; Oligonucleotide Array Sequence Analysis; Peptides - genetics; Peptides - physiology; Pheromones; Ploidies; Ploidy; Polyploidy; Ribonucleic acid; RNA; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins; Site Selection; Thallophyta, bryophyta; Transcription Factors - genetics; Vegetals; Yeast; Yeasts; Ploidy; Polyploidy; Yeast; Cyclin; Gene expression; Fungi; Regulation(control); Gene; Differential gene expression; Cell cycle; Ascomycetes; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.285.5425.251

Microarray-based gene expression analysis identified genes showing ploidy-dependent expression in isogenic Saccharomyces cerevisiae strains that varied in ploidy from haploid to tetraploid. These genes were induced or repressed in proportion to the number of chromosome sets, regardless of the mating type. Ploidy-dependent repression of some G$_1$ cyclins can explain the greater cell size associated with higher ploidies, and suggests ploidy-dependent modifications of cell cycle progression. Moreover, ploidy regulation of the FLO11 gene had direct consequences for yeast development.