Postnatal development of the mouse volatile papilla taste bud cells

• Published in: Journal of Veterinary Medical Science Vol. 65; no. 4; pp. 541 - 543
• Main Authors: Amasaki, H. (Nippon Veterinary and Animal Science Univ., Musashino, Tokyo (Japan)), Arai, R, Ogawa, M, Takemura, N, Yamagami, T, Nagasao, J, Mutoh, K, Ichihara, N, Asari, M
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.04.2003; Japan Science and Technology Agency
• Subjects: Animals; Biomarkers - analysis; CARBONATE DEHYDRATASE; carbonic anhydrase; Carbonic Anhydrases - analysis; Carbonic Anhydrases - chemistry; Carbonic Anhydrases - immunology; Female; GROWTH; gustatory cell; Immunohistochemistry - veterinary; Keratins - analysis; Keratins - immunology; Male; MICE; Mice - growth & development; mouse; Taste Buds - cytology; Taste Buds - growth & development; TONGUE
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.65.541

In the present study, we examined specific markers for taste bud cells in the mouse and the postnatal development of volatile papilla taste bud cells in ddY mice. We examined the immunoreactivity of 4 types of carbonic anhydrase isoenzymes, CA I, CA II, CA III and CA VI, as specific markers for taste bud cells, and K8.13 cytokeratin antibody as a specific marker for the lingual epithelial cells. Of the carbonic anhydrase isoenzymes, only CA III immunoreactivity was clearly detected in the spindle shaped gustatory cells. CA VI immunoreactivity was detectable in suspentacular cells. CA I and CA II antibodies did not recognize any taste bud cell specifically. K8.13 cytokeratin immunoreactivity was detected in the lingual epithelial cells, but not in taste bud cells. At 7 days after birth, the suckling phase, very small taste buds developed from the anaplastic gustatory cells. At 14 days after birth, the taste buds showed larger size than those at 7 days after birth. At 21 days birth, after the weaning phase, taste bud structure approximated the mature structure. These results demonstrate the specificity of anti-CA III and anti-CA VI for gustatory cells and suspentacular cells, respectively. These markers should be useful for an analysis of taste bud development in mice.