FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda

• Published in: Journal of Veterinary Medical Science Vol. 82; no. 2; pp. 188 - 192
• Main Authors: UCHIDA, Leo, BYARUHANGA, Joseph, OKAMURA, Ikuo, MIYAMA, Takeshi, MURAMATSU, Yasukazu, VUDRIKO, Patrick, MAKITA, Kohei
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2020; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: COI protein; Cytochrome-c oxidase; Dairy cattle; dairy cow; Deoxyribonucleic acid; DNA; East Coast fever; FTA card; Parasitology; Polymerase chain reaction; Sodium hydroxide; Theileria parva; Uganda; dairy cow; East Coast fever; sodium hydroxide; Theileria parva; FTA card
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.19-0521

East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey for T. parva prevalence was conducted in dairy cattle in Mbarara, Uganda. Cytochrome C oxidase subunit I (COI) and T. parva-p104 genes were amplified to compare the methods. FTA-NaOH-based polymerase chain reaction (PCR) yielded the best detection rate for both COI gene and p104 gene. Prevalence of T. parva was 45.0% and 83.3% at animal and farm-level, respectively. FTA-NaOH based-PCR is simple, highly sensitive and cost-effective tool for T. parva diagnosis in resource constrained settings.