Differences in Liver Injury and Trophoblastic Mitochondrial Damage in Different Preeclampsia-like Mouse Models

• Published in: Chinese medical journal Vol. 128; no. 12; pp. 1627 - 1635
• Main Authors: Han, Yi-Wei, Yang, Zi, Ding, Xiao-Yan, Yu, Huan
• Format: Journal Article
• Language: English
• Published: China Medknow Publications Pvt Ltd 20.06.2015; Medknow Publications and Media Pvt. Ltd; Lippincott Williams & Wilkins Ovid Technologies; Department of Obstetrics and Gynecology,Peking University Third Hospital,Beijing 100191,China; Medknow Publications & Media Pvt Ltd; Wolters Kluwer
• Subjects: Analysis; Animals; Apoptosis; Cholesterol - blood; Complications and side effects; Disease Models, Animal; Enzymes; Fatty acids; Female; Fetal Weight - physiology; Gene expression; Genetic aspects; Glycoproteins; Gynecology; Hospitals; Hypotheses; Laboratory animals; Lipids; Liver - injuries; Liver diseases; Male; Medical screening; Metabolism; Mice; Mice, Inbred C57BL; Mitochondria; Mitochondrial Diseases - blood; Mitochondrial Diseases - pathology; Mitochondrial DNA; Nitric oxide; Obstetrics; Original; Oxidation; Oxidative stress; Pathogenesis; Physiological aspects; Placenta; Placenta - metabolism; Pre-Eclampsia - blood; Pre-Eclampsia - pathology; Preeclampsia; Pregnancy; Proteins; Risk factors; Transgenic animals; Triglycerides - blood; Trophoblasts - pathology; Womens health; 前期; 子痫; 小鼠模型; 滋养细胞; 线粒体; 肝损伤; 血清甘油三酯; 载脂蛋白C; China; Mitochondria; Trophoblast; Triglyceride; Liver; Preeclampsia
• ISSN: 0366-6999; 2542-5641; 2542-5641
• DOI: 10.4103/0366-6999.158322

Background：Preeclampsia is a multifactorial disease during pregnancy.Dysregulated lipid metabolism may be related to some preeclampsia.We investigated the relationship between triglycerides （TGs） and liver injury in different preeclampsia-like mouse models and their potential common pathways.Methods：Preeclampsia-like models （Nw-nitro-L-arginine-methyl ester [L-NAME],lipopolysaccharide [LPS],apolipoprotein C-Ⅲ [Apo] transgnic mice ＋ L-NAME,β2 glycoprotein Ⅰ [βGPI]） were used in four experimental groups：L-NAME （LN）,LPS,Apo-LN and βGPI,respectively,and controls received saline （LN-C,LPS-C,Apo-C,βGPI-C）.The first three models were established in preimplantation （PI）,early-,mid-and late-gestation （EG,MG and LG）.βGPI and controls were injected before implantation.Mean arterial pressure （MAP）,24-hour urine protein,placental and fetal weight,serum TGs,total cholesterol （TC） and pathologic liver and trophocyte changes were assessed.Results：MAP and proteinuria were significantly increased in the experimental groups.Placenta and fetal weight in PI,EP and MP subgroups were significantly lower than LP.Serum TGs significantly increased in most groups but controls.TC was not different between experimental and control groups.Spotty hepatic cell necrosis was observed in PI,EG,MG in LN,Apo-LN and βGPI,but no morphologic changes were observed in the LPS group.Similar trophoblastic mitochondrial damage was observed in every experimental group.Conclusions：Earlier preeclampsia onset causes a higher MAP and urine protein level,and more severe placental and fetal damage.Preeclampsia-like models generated by varied means lead to different changes in lipid metabolism and associated with liver injury.Trophoblastic mitochondrial damage may be the common terminal pathway in different preeclampsia-like models.