Response to “The Reality of Pervasive Transcription”

• Published in: PLoS biology Vol. 9; no. 7; p. e1001102
• Main Authors: van Bakel, Harm, Nislow, Corey, Blencowe, Benjamin J., Hughes, Timothy R.
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 01.07.2011; Public Library of Science (PLoS)
• Subjects: Arrays; Biology; DNA microarrays; Genes; Genetic transcription; Genomes; Medical research; Physiological aspects; Ribosomal RNA; United States

[...]we demonstrate that conclusions very similar to those of our original study are reached with a dataset with far greater read depth, obtained by strand-specific sequencing of rRNA-depleted total RNA from a single cell type. [...]the mechanisms that control the positions of initiation and termina...