Response to “The Reality of Pervasive Transcription”

[...]we demonstrate that conclusions very similar to those of our original study are reached with a dataset with far greater read depth, obtained by strand-specific sequencing of rRNA-depleted total RNA from a single cell type. [...]the mechanisms that control the positions of initiation and termina...

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Bibliographic Details
Published inPLoS biology Vol. 9; no. 7; p. e1001102
Main Authors van Bakel, Harm, Nislow, Corey, Blencowe, Benjamin J., Hughes, Timothy R.
Format Journal Article
LanguageEnglish
Published San Francisco Public Library of Science 01.07.2011
Public Library of Science (PLoS)
Subjects
Arrays
Biology
DNA microarrays
Genes
Genetic transcription
Genomes
Medical research
Physiological aspects
Ribosomal RNA
United States
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Summary:[...]we demonstrate that conclusions very similar to those of our original study are reached with a dataset with far greater read depth, obtained by strand-specific sequencing of rRNA-depleted total RNA from a single cell type. [...]the mechanisms that control the positions of initiation and termination of Pol II transcription, as well as RNA processing, are imperfect, such that low-level background transcripts from both physiologically relevant and non-canonical sites arise [4]-[6].
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The Perspective section provides experts with a forum to comment on topical or controversial issues of broad interest.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.1001102