Toxicity and Selective Biochemical Assessment of Quercetin, Gallic Acid, and Curcumin in Zebrafish

• Published in: Biological & pharmaceutical bulletin Vol. 42; no. 12; pp. 1969 - 1976
• Main Authors: Harishkumar, Rajendran, Reddy, Lakki Pramod Kumar, Karadkar, Shivam H., Murad, Musa Al, Karthik, Saravanan Sibi, Manigandan, Saravanan, Selvaraj, Chinnadurai Immanuel, Christopher, JohnSamuel Godwin
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Pharmaceutical Society of Japan 01.12.2019; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Animals; Antioxidants; Biochemistry; Catalase; Catalase - metabolism; Complications; Curcumin; Curcumin - toxicity; Danio rerio; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - metabolism; Embryos; Gallic acid; Gallic Acid - toxicity; Glutathione; Glutathione - metabolism; Lethal Dose 50; Lipid peroxidation; Lipid Peroxidation - drug effects; Morphology; Oxidative stress; Peroxidation; Quercetin; Quercetin - toxicity; Superoxide dismutase; Superoxide Dismutase - metabolism; Toxicity; Zebrafish; Zebrafish - metabolism; toxicity; quercetin; zebrafish; gallic acid; Danio rerio; curcumin
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b19-00296

In recent years, numerous research outcomes were established on various naturally occurring compounds that have been shown to have beneficial antioxidant and other biological activities. Antioxidant defence mechanism plays a vital role in combating various diseases mainly due to oxidative stress. However, various models have been utilized to identify their bioactivities using these compounds (quercetin, gallic acid and curcumin). Their toxicity level also has to be explored to determine the threshold levels on the usage of these compounds. In this study, we investigated the lethal concentration of these compounds and abnormalities, biochemical and morphological changes in zebrafish embryo (Danio rerio). Toxicity level was evaluated by calculating the LD50 on the embryonic stages at 24, 48 and 72 h. Antioxidant parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and biological assays such as lipid peroxidation, protein estimation were performed. Microscopic evaluations were also observed to find out morphological abnormalities. However, these naturally derived compounds are reported to have their protective and curative role in many health complications. From the above assays, we are studying the effect of the drugs in both biochemical and molecular way in the zebrafish model organism.