Association of cdk2 Kinase with the Transcription Factor E2F During S Phase

• Published in: Science (American Association for the Advancement of Science) Vol. 255; no. 5048; pp. 1144 - 1147
• Main Authors: Pagano, Michele, Draetta, Giulio, Jansen-Dürr, Pidder
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 28.02.1992; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Adenovirus Early Proteins; Antibodies; Base Sequence; Biological and medical sciences; Carrier Proteins; CDC2-CDC28 Kinases; Cell Cycle; Cell Cycle Proteins; Cell cycle, cell proliferation; Cell extracts; Cell physiology; Cellular biology; Cyclin-Dependent Kinase 2; Cyclin-Dependent Kinases; Cyclins; Cyclins - metabolism; DNA; DNA-Binding Proteins; E2F Transcription Factors; Fundamental and applied biological sciences. Psychology; Gels; Genetic transcription; Genetics; HeLa Cells; Histones; Humans; Intellectual Disability; Interphase; Macromolecular Substances; Medical research; Molecular and cellular biology; Molecular Sequence Data; Oligodeoxyribonucleotides - chemistry; Oligonucleotides; Oncogene Proteins, Viral - genetics; Protamine Kinase - metabolism; Protein kinases; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases; Retinoblastoma-Binding Protein 1; S Phase; Transcription (Genetics); Transcription Factor DP1; Transcription Factors - metabolism; Catalytic subunit; Regulation(control); Transcription factor; Hela cell line; S Phase; Cell cycle
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1312258

The transcription factor E2F controls the expression of several proliferation-related genes and is a target of the adenovirus E1A oncogene. In human cells, both cyclin A and the cdk2 protein kinase were found in complexes with E2F. Although the total amounts of cdk2 were constant in the cell cycle, binding to E2F was detected only when cells entered S phase, a time when the cdk2 kinase is activated. These data suggest that the interaction between cdk2 and E2F requires an active kinase that has cyclin A as a targeting component.