Identification of Characteristic Phenolic Constituents in Mousouchiku Extract Used as Food Additives

• Published in: Chemical & pharmaceutical bulletin Vol. 65; no. 9; pp. 878 - 882
• Main Authors: Yoshimura, Morio, Ochi, Keisuke, Sekiya, Hiroshi, Tamai, Eiji, Maki, Jun, Tada, Atsuko, Sugimoto, Naoki, Akiyama, Hiroshi, Amakura, Yoshiaki
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 2017; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Anti-Infective Agents - chemistry; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; Bamboo; Benzoquinone; Chromatography, High Pressure Liquid; Coumaric acid; Escherichia coli - drug effects; Ferulic acid; food additive; Food additives; Food Additives - chemistry; Food Additives - isolation & purification; Furfural; High performance liquid chromatography; Liquid chromatography; lyoniresinol glucoside; Magnetic Resonance Spectroscopy; Mass Spectrometry; Microbial Sensitivity Tests; Molecular Conformation; mousouchiku extract; NMR; Nuclear magnetic resonance; p-Coumaric acid; p-Hydroxybenzoic acid; Phenolic compounds; Phenols; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Phyllostachys heterocycla; Plant Extracts - chemistry; Poaceae - chemistry; Poaceae - metabolism; propiophenone glycoside; Quinones; Staphylococcus aureus - drug effects; food additive; mousouchiku extract; Phyllostachys heterocycla; propiophenone glycoside; lyoniresinol glucoside
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.c17-00401

Mousouchiku extract is prepared from the bamboo-sheath of Phyllostachys heterocycla MITF. (Poaceae), and is registered as a food manufacturing agent in the List of Existing Food Additives in Japan. This study describes the chromatographic evaluation of characteristic components of this extract to obtain the chemical data needed for standardized specifications. We isolated 12 known compounds from this extract: 5-hydroxymethyl-2-furfural, 4-hydroxybenzoic acid, trans-p-coumaric acid, trans-ferulic acid, N,N′-diferuloylputrescine, 4′-hydroxypropiophenone, β-arbutin, tachioside, isotachioside, 3,4′-dihydroxypropiophenone 3-O-glucoside, koaburaside, and (+)-lyoniresinol 9′-O-glucoside. Moreover, a new propiophenone glycoside, propiophenone 4′-O-(6-β-D-xylosyl)-β-D-glucoside (propiophenone 4′-O-primeveroside), was isolated. The structure of each isolated compound was elucidated based on NMR and MS data or direct HPLC comparisons with authentic samples. Among the isolates, (+)-lyoniresinol 9′-O-glucoside was found to be the major ingredients of the extract as observed using HPLC analysis. However, 2,6-dimethoxy-1,4-benzoquinone, which is considered the main constituent of mousouchiku extract, was only detected as a trace constituent and not isolated in this study.