Comparisons of Effects on Intestinal Short-Chain Fatty Acid Concentration after Exposure of Two Glycosidase Inhibitors in Mice

• Published in: Biological & pharmaceutical bulletin Vol. 41; no. 7; pp. 1024 - 1033
• Main Authors: Xu, Guo-dong, Cai, Lei, Ni, Yi-shu, Tian, Shi-yi, Lu, Ying-qi, Wang, Li-na, Chen, Lian-lian, Ma, Wen-ya, Deng, Shao-ping
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Pharmaceutical Society of Japan 01.07.2018; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Acarbose; Acetic acid; Diabetes mellitus; Encyclopedias; Epithelial cells; Fatty acids; Genomes; Inhibitors; Intestinal microflora; Large intestine; Mice; Microbiomes; Microbiota; Propionic acid; Pyruvate oxidase; Pyruvic acid; short-chain fatty acid; Starch; Tax4Fun; voglibose; microbiota; short-chain fatty acid; voglibose; Tax4Fun; acarbose
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b17-00978

Acarbose and voglibose are the most widely used diabetes drugs as glycosidase inhibitors. In this study, the use of these two inhibitors significantly increased the content of starch in large intestine, and altered the concentration of short-chain fatty acids (SCFAs) by affecting the intestinal microbiota. However, there are some differences in the intestinal microbiome of the two groups of mice, mainly in bacteria such as Bacteroidaceae bacteroides and Desulfovibrionaceae desulfovibrio. The productions of acetate and propionate in caecum in voglibose group were significantly higher than those in acarbose group and two kinds of glycosidase inhibitors were close in the production of butyrate in caecum. The Tax4Fun analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data indicated that different productions of acetate and propionate between acarbose group and voglibose group may be related to 2-oxoisovalerate dehydrogenase and pyruvate oxidase. In addition, in-vitro experiments suggested that voglibose had less effect on epithelial cells than acarbose after direct stimulation. According to the recent researches of SCFAs produced by intestinal microbiota, our comparative study shown higher concentration of these beneficial fatty acids in the lumen of voglibose-treated mice, which implied a lower level of inflammation.