Interleukin 18 polymorphisms and its serum level in patients with multiple sclerosis

• Published in: Annals of the Indian Academy of Neurology Vol. 22; no. 4; pp. 474 - 476
• Main Authors: Jahanbani-Ardakani, Hamidreza, Alsahebfosoul, Fereshteh, Etemadifar, Masoud, Abtahi, Seyed-Hossein
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01.10.2019; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Wolters Kluwer - Medknow; Wolters Kluwer Medknow Publications
• Subjects: Arthritis; Central nervous system; Cytokines; Demyelination; Diagnosis; Disease; Enzyme-linked immunosorbent assay; Genes; Genetic aspects; Genetic polymorphisms; Genotype & phenotype; Health aspects; Interleukin 18; Interleukins; Medical research; Multiple sclerosis; Pathogenesis; Polymerase chain reaction; Polymorphism; Risk factors; serum level; Serum levels; Short Communications; single-nucleotide polymorphism; Studies; India; United States > US; Chicago Illinois; California; serum level; Multiple sclerosis; single-nucleotide polymorphism; interleukin 18
• ISSN: 0972-2327; 1998-3549
• DOI: 10.4103/aian.AIAN_515_18

Background: Multiple sclerosis (MS) is a chronic demyelinating disorder of central nervous system. Although the definite pathogenesis of MS has not been understood, crucial role of environmental and genetic risk factors has been proposed. Propose: To determine the serum level of interleukin-18 (IL-18) as well as gene polymorphisms of IL-18 (rs1946518, rs360719, and rs187238). Methods: In this case-control study, 110 MS patients diagnosed according to the McDonald criteria and 110 healthy individuals were recruited. IL-18 gene polymorphisms were genotyped by polymerase chain reaction high-resolution melt test, and IL-18 serum level was determined by enzyme-linked immunosorbent assay technique. Results: The mean age of the MS patients (89 females and 21 males) and the control group (89 females and 21 males) was 30.3 ± 9.25 and 30.28 ± 9.13 years, respectively. The mean serum levels of IL-18 in MS patients and healthy individuals were 341.56 ± 39.22 Pg/Ml and 146.52 ± 29.30 Pg/Ml, respectively (P < 0.001). The genotype of rs1946518 (but not rs360719 and rs187238) was significantly different between groups (P = 0.037 and P = 0.069, respectively). Conclusion: In this study, we showed the significant higher IL-18 serum level and significant different frequencies of two polymorphisms of IL-18 in MS patients. These results show the important roles of IL-18 in MS pathogenesis. However, more studies are needed to verify our results in larger sample size.