Promotion of lipopeptide antibiotic production by Bacillus sp. IA in the presence of rice husk biochar

• Published in: Journal of Pesticide Science Vol. 44; no. 1; pp. 33 - 40
• Main Authors: Ebe, Shohei, Ohike, Tatsuya, Matsukawa, Tetsuya, Okanami, Masahiro, Kajiyama, Shin’ichiro, Ano, Takashi
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Pesticide Science Society of Japan 20.02.2019; Japan Science and Technology Agency
• Subjects: Activated carbon; Activated charcoal; Antibiotics; Antifungal activity; Bacillus; bacterial growth enhancement; biocontrol agent; biofertilizer; Charcoal; Fungicides; High-performance liquid chromatography; Iturin A; iturin A production; Liquid chromatography; Microorganisms; Original; Oryza; Probiotics; rice husk biochar; Sporulation; Strain analysis; swarming motility; biofertilizer; bacterial growth enhancement; iturin A production; swarming motility; biocontrol agent; rice husk biochar
• ISSN: 1348-589X; 1349-0923
• DOI: 10.1584/jpestics.D18-042

The purpose of this study is to isolate the beneficial microorganisms whose growth is promoted in the presence of charcoal materials. We successfully isolated strain IA, whose growth is promoted on an agar plate with charcoal materials, and identified it as a novel strain of the Bacillus sp. The growth of strain IA in the liquid medium was promoted by the addition of both activated charcoal (AC) and rice husk biochar (RHB). Moreover, the sporulation of strain IA in the RHB medium and the antifungal activity of the culture supernatant of the RHB medium were much higher than those with AC. HPLC and MS analyses revealed that strain IA produced an antifungal lipopeptide iturin A, and the yield of iturin A in the RHB medium was 8 times higher than that in the medium without RHB. This is the first paper to describe the positive effect of RHB on microbial metabolisms.