Host microbiota constantly control maturation and function of microglia in the CNS

• Published in: Nature neuroscience Vol. 18; no. 7; pp. 965 - 977
• Main Authors: Erny, Daniel, Hrabě de Angelis, Anna Lena, Jaitin, Diego, Wieghofer, Peter, Staszewski, Ori, David, Eyal, Keren-Shaul, Hadas, Mahlakoiv, Tanel, Jakobshagen, Kristin, Buch, Thorsten, Schwierzeck, Vera, Utermöhlen, Olaf, Chun, Eunyoung, Garrett, Wendy S, McCoy, Kathy D, Diefenbach, Andreas, Staeheli, Peter, Stecher, Bärbel, Amit, Ido, Prinz, Marco
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.07.2015; Nature Publishing Group
• Subjects: 14/19; 14/63; 42/35; 42/44; 631/378/1457/1936; 631/378/1689/1414; 631/378/1831; 631/378/3920; 64/110; 9/74; Analysis; Animal Genetics and Genomics; Animals; Behavioral Sciences; Biological Techniques; Biomedicine; Brain research; Cell cycle; Central Nervous System - immunology; Central Nervous System - metabolism; Central Nervous System - physiology; Disease; Fatty Acids, Volatile - immunology; Fatty Acids, Volatile - metabolism; Female; Homeostasis; Homeostasis - immunology; Homeostasis - physiology; Hygiene; Immunity, Innate - immunology; Immunity, Innate - physiology; Immunology; Male; Mice; Mice, Inbred C57BL; Microbiota; Microbiota (Symbiotic organisms); Microbiota - immunology; Microbiota - physiology; Microglia - immunology; Microglia - metabolism; Microglia - physiology; Neurobiology; Neurosciences; Receptors, G-Protein-Coupled - deficiency; Germany
• ISSN: 1097-6256; 1546-1726; 1546-1726
• DOI: 10.1038/nn.4030

In this study, the authors show that host microbiota play a key role in modulating microglia homeostasis. Germ-free mice or mice with only limited microbiota complexity displayed defects in microglial cell proportions and maturation, leading to impaired innate immune responses. The authors find that short-chain fatty acid signaling regulates these effects in vivo . As the tissue macrophages of the CNS, microglia are critically involved in diseases of the CNS. However, it remains unknown what controls their maturation and activation under homeostatic conditions. We observed substantial contributions of the host microbiota to microglia homeostasis, as germ-free (GF) mice displayed global defects in microglia with altered cell proportions and an immature phenotype, leading to impaired innate immune responses. Temporal eradication of host microbiota severely changed microglia properties. Limited microbiota complexity also resulted in defective microglia. In contrast, recolonization with a complex microbiota partially restored microglia features. We determined that short-chain fatty acids (SCFA), microbiota-derived bacterial fermentation products, regulated microglia homeostasis. Accordingly, mice deficient for the SCFA receptor FFAR2 mirrored microglia defects found under GF conditions. These findings suggest that host bacteria vitally regulate microglia maturation and function, whereas microglia impairment can be rectified to some extent by complex microbiota.