Dynamic Disruptions in Nuclear Envelope Architecture and Integrity Induced by HIV-1 Vpr

• Published in: Science (American Association for the Advancement of Science) Vol. 294; no. 5544; pp. 1105 - 1108
• Main Authors: Carlos M. C. de Noronha, Sherman, Michael P., Lin, Harrison W., Cavrois, Marielle V., Moir, Robert D., Goldman, Robert D., Greene, Warner C.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 02.11.2001; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Active Transport, Cell Nucleus; Biological and medical sciences; cdc25 Phosphatases - metabolism; Cell Cycle Proteins - metabolism; Cell nucleus; Cell Nucleus - metabolism; Cell Nucleus - virology; Cell research; Cellular biology; Cyclin B - metabolism; Cyclin B1; Cyclins; Cytological research; Cytoplasm - metabolism; Daughter cells; Fundamental and applied biological sciences. Psychology; G2 Phase; Gene Products, vpr - genetics; Gene Products, vpr - physiology; HeLa Cells; HIV; HIV (Viruses); HIV 1; HIV-1 - physiology; Human immunodeficiency virus; Human immunodeficiency virus 1; Humans; Interphase; Lamin Type B; Lamins; Macrophages - virology; Microbiology; Microscopy, Fluorescence; Microscopy, Video; Mitosis; Molecular biology; Mutation; Nuclear Envelope - metabolism; Nuclear Envelope - ultrastructure; Nuclear lamina; Nuclear Pore Complex Proteins - metabolism; Nuclear Proteins - metabolism; Protein-Tyrosine Kinases - metabolism; Proteins; Recombinant Fusion Proteins - metabolism; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Transfection; United States; Virology; Virus Integration; vpr Gene Products, Human Immunodeficiency Virus; vpr protein; United States; Virus; Growing phase; Cell culture; HIV-1 virus; Mitosis; Retroviridae; Cell division; G2 Phase; Human immunodeficiency virus; Lentivirus; Host virus relation
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1063957

Human immunodeficiency virus-1 (HIV-1) Vpr expression halts the proliferation of human cells at or near the G2cell-cycle checkpoint. The transition from G2to mitosis is normally controlled by changes in the state of phosphorylation and subcellular compartmentalization of key cell-cycle regulatory proteins. In studies of the intracellular trafficking of these regulators, we unexpectedly found that wild-type Vpr, but not Vpr mutants impaired for G2arrest, induced transient, localized herniations in the nuclear envelope (NE). These herniations were associated with defects in the nuclear lamina. Intermittently, these herniations ruptured, resulting in the mixing of nuclear and cytoplasmic components. These Vpr-induced NE changes probably contribute to the observed cell-cycle arrest.