Determination of rhizosphere 13 C pulse signals in soil thin sections by laser ablation isotope ratio mass spectrometry
Abstract In grassland ecosystems, soil animals act as key soil engineers and architects. The diversity of soil animals is also a regulator of ecosystem carbon flow. However, our understanding of the link between soil animals, carbon fluxes and soil physical organisation remains poor. An integrated a...
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Published in | Rapid communications in mass spectrometry Vol. 16; no. 23; pp. 2190 - 2194 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
15.12.2002
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Online Access | Get full text |
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Summary: | Abstract
In grassland ecosystems, soil animals act as key soil engineers and architects. The diversity of soil animals is also a regulator of ecosystem carbon flow. However, our understanding of the link between soil animals, carbon fluxes and soil physical organisation remains poor. An integrated approach based on soil micromorphology and laser ablation stable isotope ratio mass spectrometry (LA‐IRMS) was developed to provide spatially distributed data of pulse‐derived
13
C tracer from roots in the soil environment. This paper describes the development and testing of a LA‐IRMS
13
C/
12
C analytical method on soil thin sections as a means to determine the fate of root carbon derived from photosynthesis into soil. Results from this work demonstrated (1) that micro‐scale δ
13
C (‰) analysis could be made on targeted features located within a soil thin section and (2) that LA‐IRMS δ
13
C (‰) measurements made on samples obtained from
13
CO
2
pulse labelled plant‐soil blocks confirmed the presence of recent photosynthates in the rhizosphere (1 and 4 weeks post‐pulse). Copyright © 2002 John Wiley & Sons, Ltd. |
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ISSN: | 0951-4198 1097-0231 |
DOI: | 10.1002/rcm.740 |