Recombinant, rice-produced yeast phytase shows the ability to hydrolyze phytate derived from seed-based feed, and extreme stability during ensilage treatment

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 6; pp. 1524 - 1527
• Main Authors: Hamada, A.(Mitsui Chemicals Inc., Mobara, Chiba (Japan)), Yamaguchi, K, Harada, M, Horiguchi, K, Takahashi, T, Honda, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.06.2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 6-Phytase - genetics; 6-Phytase - metabolism; ADDITIF AUX ALIMENTS DES ANIMAUX; ADITIVOS DE PIENSOS; Biological and medical sciences; biotechnology; ENSILADO; ensilage; ENSILAGE (PRODUIT); enzyme activity; Enzyme Stability; feed additive; FEED ADDITIVES; FITASA; Food Handling; Fundamental and applied biological sciences. Psychology; Hydrolysis; LEVADURA; LEVURE; orthophosphates; Oryza - genetics; ORYZA SATIVA; phosphorus; PHYTASE; phytases; phytic acid; Phytic Acid - metabolism; phytin; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Stems - chemistry; Plant Stems - enzymology; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants, Genetically Modified; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; rice; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Schwanniomyces occidentalis; Seeds - chemistry; SILAGE; Temperature; Time Factors; TRANSGENIC PLANTS; YEASTS; Monocotyledones; Stability; Enzyme; 6-Phytase; Phosphoric monoester hydrolases; ensilage; feed additive; phytin; Esterases; Genetically modified microorganism; Hydrolysis; Oryza sativa; phytase; Gramineae; Angiospermae; Hydrolases; Spermatophyta; Rice

When fresh rice leaves producing yeast Schwanniomyces occidentalis phytase were grounded and mixed with the whole extract of seed-based feed for pigs, the release of orthophosphate increased significantly. More specifically, phytate, a major source of phosphorus in the seeds, was hydrolyzed by heter...