Recombinant, rice-produced yeast phytase shows the ability to hydrolyze phytate derived from seed-based feed, and extreme stability during ensilage treatment

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 6; pp. 1524 - 1527
• Main Authors: Hamada, A.(Mitsui Chemicals Inc., Mobara, Chiba (Japan)), Yamaguchi, K, Harada, M, Horiguchi, K, Takahashi, T, Honda, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.06.2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 6-Phytase - genetics; 6-Phytase - metabolism; ADDITIF AUX ALIMENTS DES ANIMAUX; ADITIVOS DE PIENSOS; Biological and medical sciences; biotechnology; ENSILADO; ensilage; ENSILAGE (PRODUIT); enzyme activity; Enzyme Stability; feed additive; FEED ADDITIVES; FITASA; Food Handling; Fundamental and applied biological sciences. Psychology; Hydrolysis; LEVADURA; LEVURE; orthophosphates; Oryza - genetics; ORYZA SATIVA; phosphorus; PHYTASE; phytases; phytic acid; Phytic Acid - metabolism; phytin; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Stems - chemistry; Plant Stems - enzymology; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants, Genetically Modified; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; rice; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Schwanniomyces occidentalis; Seeds - chemistry; SILAGE; Temperature; Time Factors; TRANSGENIC PLANTS; YEASTS; Monocotyledones; Stability; Enzyme; 6-Phytase; Phosphoric monoester hydrolases; ensilage; feed additive; phytin; Esterases; Genetically modified microorganism; Hydrolysis; Oryza sativa; phytase; Gramineae; Angiospermae; Hydrolases; Spermatophyta; Rice
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.60039

When fresh rice leaves producing yeast Schwanniomyces occidentalis phytase were grounded and mixed with the whole extract of seed-based feed for pigs, the release of orthophosphate increased significantly. More specifically, phytate, a major source of phosphorus in the seeds, was hydrolyzed by heterologous phytase. Moreover, when transgenic rice plants were ensiled for up to 12 weeks, no decrease in the phytase activity of the heterologous enzyme was observed. This result strongly suggests that transgenic rice plants producing yeast phytase can be stored as silage without any loss of enzyme activity until usage as a feed additive.