A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects

• Published in: PLoS pathogens Vol. 10; no. 2; p. e1003829
• Main Authors: Mutlu, Ece A., Keshavarzian, Ali, Losurdo, John, Swanson, Garth, Siewe, Basile, Forsyth, Christopher, French, Audrey, DeMarais, Patricia, Sun, Yan, Koenig, Lars, Cox, Stephen, Engen, Phillip, Chakradeo, Prachi, Abbasi, Rawan, Gorenz, Annika, Burns, Charles, Landay, Alan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.02.2014; Public Library of Science (PLoS)
• Subjects: Antiretroviral drugs; Bacteria; Development and progression; Disease; Female; Gastrointestinal system; High-Throughput Nucleotide Sequencing; Highly active antiretroviral therapy; HIV; HIV (Viruses); HIV infection; HIV Infections - immunology; HIV Infections - microbiology; Human immunodeficiency virus; Humans; Illnesses; Immune system; Intestinal Mucosa - immunology; Intestinal Mucosa - microbiology; Lymphocytes; Male; Medical research; Medicine; Medicine, Experimental; Microbiota; Microbiota (Symbiotic organisms); Middle Aged; Mortality; Physiological aspects; Reverse Transcriptase Polymerase Chain Reaction; RNA, Ribosomal, 16S - analysis; HIV Infections; Intestinal Mucosa; Microbiota; Humans; Middle Aged; Female; Male; High-Throughput Nucleotide Sequencing; RNA, Ribosomal, 16S; Reverse Transcriptase Polymerase Chain Reaction
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1003829

HIV progression is characterized by immune activation and microbial translocation. One factor that may be contributing to HIV progression could be a dysbiotic microbiome. We therefore hypothesized that the GI mucosal microbiome is altered in HIV patients and this alteration correlates with immune activation in HIV. 121 specimens were collected from 21 HIV positive and 22 control human subjects during colonoscopy. The composition of the lower gastrointestinal tract mucosal and luminal bacterial microbiome was characterized using 16S rDNA pyrosequencing and was correlated to clinical parameters as well as immune activation and circulating bacterial products in HIV patients on ART. The composition of the HIV microbiome was significantly different than that of controls; it was less diverse in the right colon and terminal ileum, and was characterized by loss of bacterial taxa that are typically considered commensals. In HIV samples, there was a gain of some pathogenic bacterial taxa. This is the first report characterizing the terminal ileal and colonic mucosal microbiome in HIV patients with next generation sequencing. Limitations include use of HIV-infected subjects on HAART therapy.