Uniform Binding of Aminoacyl-tRNAs to Elongation Factor Tu by Thermodynamic Compensation

• Published in: Science (American Association for the Advancement of Science) Vol. 294; no. 5540; pp. 165 - 168
• Main Authors: LaRiviere, Frederick J., Wolfson, Alexey D., Uhlenbeck, Olke C.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 05.10.2001; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Acylation; Amino acids; Amino Acids - chemistry; Amino Acids - metabolism; aminoacyl-tRNA; Biochemistry; Biological and medical sciences; Carrier proteins; Cells; Cellular biology; elongation factor EF-Tu; Escherichia coli - metabolism; Esterification; Evolution, Molecular; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Nucleotides; Peptide Biosynthesis; Peptide Elongation Factor Tu - metabolism; Peptide elongation factors; Protein Binding; Protein Biosynthesis; Proteins; Ribonucleic acid; Ribosomes; Ribosomes - metabolism; RNA; RNA, Transfer, Amino Acid-Specific - chemistry; RNA, Transfer, Amino Acid-Specific - metabolism; RNA, Transfer, Amino Acyl - chemistry; RNA, Transfer, Amino Acyl - metabolism; Studies; Temperature; Thermodynamics; Thermus thermophilus - metabolism; Transfer RNA; Transport proteins; Yeasts; Yeasts - metabolism; United States
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1064242

Elongation factor Tu (EF-Tu) binds all elongator aminoacyl-transfer RNAs (aa-tRNAs) for delivery to the ribosome during protein synthesis. Here, we show that EF-Tu binds misacylated tRNAs over a much wider range of affinities than it binds the corresponding correctly acylated tRNAs, suggesting that the protein exhibits considerable specificity for both the amino acid side chain and the tRNA body. The thermodynamic contributions of the amino acid and the tRNA body to the overall binding affinity are independent of each other and compensate for one another when the tRNAs are correctly acylated. Because certain misacylated tRNAs bind EF-Tu significantly more strongly or weakly than cognate aa-tRNAs, EF-Tu may contribute to translational accuracy.