Canna starch improves immune functions and the intestinal environment in mice

• Published in: Bioscience of Microbiota, Food and Health Vol. 42; no. 2; pp. 2022-068 - 137
• Main Authors: TANAKA, Mamoru, KOIDA, Ayaka, MIYAZAKI, Akira, TABATA, Kazushi, TAKEI, Yuichiro, TANIMOTO, Yoshihumi, KAWAMURA, Mami, TSUZUKI, Masafumi, TAKAHASHI, Haruka, YANO, Tetsu, WATANABE, Hiroyuki
• Format: Journal Article
• Language: English; Japanese
• Published: Japan BMFH Press 01.01.2023; Japan Science and Technology Agency
• Subjects: Acetic acid; Amylose; Butyric acid; canna starch; cecal organic acids; Cecum; commensal microbe; Diet; immunoglobulin; Immunoglobulin A; Intestinal microflora; Intestine; Lactic acid; Lymphocytes; mucins; Organic acids; resistant starch; Starch; Starches; commensal microbe; immunoglobulin; canna starch; cecal organic acids; mucins; resistant starch
• ISSN: 2186-6953; 2186-3342; 2186-3342
• DOI: 10.12938/bmfh.2022-068

The present study was conducted to elucidate the dietary effects of canna starch on the immune functions and intestinal luminal environment in mice. The amylose and resistant starch characteristics were determined for six types of starch, including edible canna. Canna starch was found to be higher in amylose and resistant starch compared with the other starches. BALB/c mice were fed 3.16% (low-canna group) and 6.32% (high-canna group) canna starch for 2 weeks, and then intestinal parameters were measured. Fecal IgA and mucin levels were markedly elevated by canna starch intake. IgA levels in serum and spleen lymphocytes were elevated by canna starch intake in the high-canna group, but not in the low-canna group. When the mice were fed canna starch, the cecum weight increased, and the pH in the cecum decreased. The high-canna group had significantly increased levels of Clostridium subcluster XIVa lactic acid, acetic acid, and n-butyric acid in the cecum compared with the control group. These results suggested that canna starch supplementation changed the intestinal microbiota and enhanced the intestinal immune and barrier functions and cecal organic acids in mice.