Cloning of the Chromosome Breakpoint of Neoplastic B Cells with the t(14;18) Chromosome Translocation

• Published in: Science (American Association for the Advancement of Science) Vol. 226; no. 4678; pp. 1097 - 1099
• Main Authors: Tsujimoto, Yoshihide, Finger, Lawrence R., Yunis, Jorge, Nowell, Peter C., Croce, Carlo M.
• Format: Journal Article
• Language: English
• Published: Washington, DC The American Association for the Advancement of Science 30.11.1984; American Association for the Advancement of Science
• Subjects: Animals; B cell leukemia; B cell lymphoma; B lymphocytes; B-Lymphocytes - cytology; Biological and medical sciences; Cancer; Cancer research; Cell lines; Chromosome Banding; Chromosome translocation; Chromosomes; Chromosomes, Human, 13-15; Chromosomes, Human, 16-18; Cloning; Cloning, Molecular; Cricetinae; Cricetulus; DNA probes; DNA Restriction Enzymes; DNA, Recombinant - analysis; Genetic loci; Genetic research; Germ cells; Hematologic and hematopoietic diseases; Human chromosomes; Humans; Hybrid Cells - cytology; Karyotyping; Leukemia - genetics; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Mice; Oncology, Experimental; Translocation (Genetics); Translocation, Genetic; Translocations (Genetics); Chromosomal aberration; Abnormal D14 chromosome; Human; Cell culture; Leukemia; Abnormal E18 chromosome; B-Lymphocyte; Cell line; Abnormal chromosome; Tumor; Molecular cloning; Physical map; Chromosome translocation
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.6093263

From an acute B-cell leukemia cell line, a DNA probe was obtained that was specific for chromosome 18 and flanked the heavy chain joining region of the immunoglobulin heavy chain locus on chromosome 14. This probe detected rearrangement of the homologous DNA segment in the leukemic cells and in follicular lymphoma cells with the t(14;18) chromosome translocation but not in other neoplastic or normal B or T cells. The probe appears to identify bcl-2, a gene locus on chromosome 18 (band q21) that is unrelated to known oncogenes and may be important in the pathogenesis of B-cell neoplasms with this translocation.