Recent Advances in the Polymerase Chain Reaction

The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from th...

Full description

Saved in:
Bibliographic Details
Published inScience (American Association for the Advancement of Science) Vol. 252; no. 5013; pp. 1643 - 1651
Main Authors Erlich, Henry A., Gelfand, David, Sninsky, John J.
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for the Advancement of Science 21.06.1991
American Association for the Advancement of Science
The American Association for the Advancement of Science
Subjects
Analysis
Annealing
Binding Sites
Biological and medical sciences
Biological Evolution
Biotechnology
Cellular biology
DNA
DNA - genetics
DNA Fingerprinting
DNA probes
Enzymes
Forensic Medicine - methods
Frontiers in Biotechnology
Fundamental and applied biological sciences. Psychology
Gene Expression
Genetic Diseases, Inborn - genetics
Genetic engineering
Genetic mutation
Genetic technics
Genetics
Genomics
Human Genome Project
Humans
Methods. Procedures. Technologies
Miscellaneous
Molecular genetics
Multigene Family
Mutation
Nucleotide sequences
Oligonucleotides - chemistry
Polymerase Chain Reaction
Product category rules
reviews
Sequencing
Synthetic digonucleotides and genes. Sequencing
Polymerase chain reaction
Gene amplification
Technical progress
Instruments
Method
Reaction specificity
Medical application
Contamination
Novelty
Online AccessGet full text

Cover

More Information
Summary:The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences. PCR can provide insights into the intricacies of single cells as well as the evolution of species. Some recent developments in instrumentation, methodology, and applications of the PCR are presented in this review.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
ObjectType-Review-3
content type line 23
ObjectType-Feature-3
ObjectType-Review-1
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2047872