Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors

• Published in: Applied microbiology and biotechnology Vol. 91; no. 4; pp. 1215 - 1225
• Main Authors: Zhang, Tong, Ye, Lin, Tong, Amy Hin Yan, Shao, Ming-Fei, Lok, Si
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2011; Springer; Springer Nature B.V
• Subjects: Abundance; Ammonia; Ammonia - metabolism; Analysis; Archaea; Archaea - classification; Archaea - genetics; Archaea - metabolism; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biodiversity; Biological and medical sciences; Biological treatment of waters; Bioreactors; Bioreactors - microbiology; Biotechnology; Cloning; Cluster Analysis; DNA, Archaeal - chemistry; DNA, Archaeal - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Environment and pollution; Environmental Biotechnology; Fundamental and applied biological sciences. Psychology; Genes; Genes, rRNA; Industrial applications and implications. Economical aspects; Laboratories; Life Sciences; Membrane reactors; Methods. Procedures. Technologies; Microbial Genetics and Genomics; Microbiology; Microorganisms; Molecular Sequence Data; Nitrates; Nitrification; Oxidation; Oxidation-Reduction; Oxidoreductases - genetics; Phylogenetics; Phylogeny; Proteins; Purification; Ribonucleic acid; RNA; RNA, Archaeal - genetics; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sewage; Sludge; Studies; Thermal cycling; Various methods and equipments; Water Microbiology; Water Purification; Water treatment; Water treatment plants; China; Ammonia-oxidizing archaea; Municipal wastewater treatment plants; Ammonia-oxidizing bacteria; 454 Pyrosequencing; Archaeobacteria; Biological purification; Ammonia; Bioreactor; ammonia oxidizing bacteria; Bacteria; Urban waste water; Oxidation; Waste water purification
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-011-3408-y

In this study, dideoxy sequencing and 454 high-throughput sequencing were used to analyze diversities of the ammonia monooxygenas e ( amo A) genes and the 16S rRNA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in six municipal wastewater treatment plants. The results showed that AOB amo A genes were quite diverse in different wastewater treatment plants while the 16S rRNA genes were relatively conserved. Based on the observed complexity of amo A and 16S rRNA genes, most of the AOB can be assigned to the Nitrosomonas genus, with Nitrosomonas ureae , Nitrosomonas oligotropha , Nitrosomonas marina , and Nitrosomonas aestuarii being the four most dominant species. From the sequences of the AOA amo A genes, most AOA observed in this study belong to the CGI.1b group, i.e., the soil lineage. The AOB amo A and 16S rRNA genes were quantified by quantitative PCR and 454 high-throughput pyrosequencing, respectively. Although the results from the two approaches show some disconcordance, they both indicated that the abundance of AOB in activated sludge was very low.